Propargyl-PEG8-bromide is a PEG reagent containing an alkyne group and a bromide group. The alkyne group can react with azide-bearing compounds or biomolecules in Click Chemistry reactions under the catalyzation of copper. The PEG spacer increases water-solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG8-bromide is a PEG reagent containing an alkyne group and a bromide group. The alkyne group can react with azide-bearing compounds or biomolecules in Click Chemistry reactions under the catalyzation of copper. The PEG spacer increases water-solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Synovial fluid is secreted into the joint cavity from the inner membrane of synovial joints. Synovial fluid is a plasma ultrafiltrate which contains proteins derived from both the blood plasma and produced by cells within the joint tissues. It lubricates the articulating joints as well as supplies oxygen and nutrients while removing carbon dioxide and metabolic wastes from the chondrocytes in the surrounding cartilage. Septic arthritis is usually caused by bacterial, viral or fungal infections to the synovial fluid. Staphylococcus aureus is the most common bacterial infection causing Septic arthritis. Diagnosing Septic arthritis is done through joint fluid (synovial fluid) analysis, blood tests or imaging tests.
Norgen’s Synovial Fluid Bacterial Genomic DNA Isolation Kit provides a fast, reliable and simple procedure for isolating the highest quality and the highest quantity of bacterial genomic DNA from various amounts of synovial fluid ranging from 1 mL up to 10 mL. Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all Gram +ve and Gram -ve strains. Moreover, the kit allows the user to elute the purified bacterial genomic DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified bacterial gDNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern Blot analysis and NGS.
Figure 1 / 2
Click for expanded view
| Kit Specifications | |
| Maximum Input | 2 x 109 bacterial cells |
| Column Binding Capacity | 25 μg |
| Average Yield | up to 20 µg |
| Time to Complete 10 Purifications | 45 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 67900 (50 preps) |
|---|---|
| Precipitation Solution B | 15 mL |
| Lysis Buffer T | 6.5 mL |
| Solution BX | 28 mL |
| Wash Solution A | 38 mL |
| Elution Buffer B | 8 mL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Propargyl-PEG12-amine is a heterobifunctional reagent that enables amide formation with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group is reactive with azide compounds via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The PEG units enhance the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG12-amine is a heterobifunctional reagent that enables amide formation with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group is reactive with azide compounds via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The PEG units enhance the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
SKU: MTP96
The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit
The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit
