Propargyl-PEG9-bromide consists of a propargyl group and a bromide group. The propargyl group reacts with azide compounds in copper catalyzed Click Chemistry reactions. The bromide (Br)can be used as a leaving group for nucleophilic substitution reactions. The 9-unit PEG spacer improves the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG9-bromide consists of a propargyl group and a bromide group. The propargyl group reacts with azide compounds in copper catalyzed Click Chemistry reactions. The bromide (Br)can be used as a leaving group for nucleophilic substitution reactions. The 9-unit PEG spacer improves the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
TDL5B refrigerated low speed centrifuge
Technical Parameters
| Max speed： | 5000rpm | Max RCF： | 4730xg |
| Max volume | 4x800ml | Noise： | ≤65dBA |
| Timer | 1～99h59min | Net weight： | 125 Kg |
| Dimension(HxDxW)： | 430×688×715mm | Power supply | AC 220V 50HZ 15A |
| Temperature range | -20℃~40℃ | Temperature accuracy | ±1℃ |
| Speed accuracy： | ±20rpm | Package | wooden box |
Matched Rotors for TDL5B
| Order No. | Model | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
| No.30671 | Swing out rotor | 4000 | 4x800ml (round cup) | 3450 |
| Adapters for 4x800ml (round cup) swing rotor for 5ml, 7ml, 10ml, 15ml, 50ml, 100ml, 250ml, 500ml tubes | ||||
| No.30696 | Swing out rotor | 4000 | 4x500ml (square cup) | 3310 |
| No.31277 | Swing out rotor body | |||
| Matched Buckets for the swing out rotor body | 5000 | 4x50ml | 4420 | |
| 4x100ml | ||||
| 4000 | 8x50ml | 2950 | ||
| 16x15ml | ||||
| 24x15ml | ||||
| 4000 | 24×10/7ml vacuum tube | 2580 | ||
| 16x10ml vacuum tube | ||||
| Order No. | Model | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
| No.31377 | Matched Buckets for the swing out rotor body | 5000 | 32x10ml vacuum tube | 2580 |
| 32x7ml vacuum tube adapter | ||||
| 32x5ml vacuum tube adapter | ||||
| 24x10ml vacuum tube | 2580 | |||
| 24x7ml vacuum tube adapter | ||||
| 24x5ml vacuum tube adapter | ||||
| 16x10ml vacuum tube | 2580 | |||
| 16x7ml vacuum tube adapter | ||||
| 16x5ml vacuum tube ada |
N-(Aminooxy-PEG2)-N-bis(PEG3-propargyl) consists of two terminal propargyl groups and an aminooxy group. The propargyl groups reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The aminooxy group is reactive towards aldehydes to form oxime bonds. If a reductant is used, it will form a hydroxylamine linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
N-(Aminooxy-PEG2)-N-bis(PEG3-propargyl) consists of two terminal propargyl groups and an aminooxy group. The propargyl groups reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The aminooxy group is reactive towards aldehydes to form oxime bonds. If a reductant is used, it will form a hydroxylamine linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for small quantities of tissue (<100mg) and cells (<5 X106), and large quantities of tissue (up to 1g) and cells (<108), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.
Specifications
| Features | Specifications |
| Main Functions | Extract RNA from liquid samples by salting out method |
| Applications | RT-PCR, Northern hybridization, poly (a) enrichment, etc. |
| Purification technology | Acid phenol guanidine isothiocyanate |
| Process method | Manual (centrifugation) |
| Sample type | Various liquid samples |
| Sample amount | Flexible |
| Elution volume | Variation with sample size |
| Time per run | Variation with sample size |
Storage and Stability
MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance.
MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
