Alkyne-PEG2-iodide is a crosslinker containing a propargyl group and an iodine group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. Iodine (I) is a very good leaving group for nucleophilic substitution reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
Alkyne-PEG2-iodide is a crosslinker containing a propargyl group and an iodine group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. Iodine (I) is a very good leaving group for nucleophilic substitution reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
Usages:
For selective isolation and culture of Pseudomonas aeruginosa.
Principle:
Acetamidase bacteria can use acetamide as a carbon source and acetamide decomposition Alcaligenes; NaCl maintain osmotic balance; dipotassium phosphate and potassium dihydrogenphosphate as buffer; magnesium ions provide necessary growth ; agar medium as coagulant; phenol red as a pH indicator.
Formulation:
Acetamide 10g
NaCl 5g
Dipotassium hydrogen phosphate 1.39g
Potassium dihydrogen phosphate 0.73g
Magnesium sulfate 0.5g
Phenol red 0.012g
Agar 15g
Final pH 7.2 ± 0.2
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
100g
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
Specifications
| Features | Specifications |
| Main Functions | Co-isolation DNA and RNA from FFPE tissue |
| Applications | RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc. |
| Purification method | Polydisperse silicon based magnetic beads (DNA)Monodisperse carbonyl magnetic beads (RNA) |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor and pipetting workstation |
| Sample type | FFPE slice, FFPE puncture sample, embedded tissue |
| Sample amount | No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area |
| Yield | DNA: 1 – 10 μg, RNA: 1 – 25 μg |
The sample is lysed and digested under the action of lysate and protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, the supernatant contain RNA was collected and bind to MagBind Particles. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Elution Buffer.
Advantages
Kit Contents
| Contents | R632701 | R632702 | R632703 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagBind Particles | 1.1 ml | 2.5 ml | 11 ml |
| MagPure Particles N | 1.1 ml | 2.5 ml | 11 ml |
| Proteinase K | 24 mg | 48 mg | 220 mg |
| Protease Dissolve Buffer | 3 ml | 10 ml | 15 ml |
| Buffer DPS | 50 ml | 100 ml | 2 x 250 ml |
| Buffer ATL | 20 ml | 30 ml | 120 ml |
| Buffer BST1 | 20 ml | 40 ml | 200 ml |
| Buffer BXW1* | 44 ml | 110 ml | 3 x 110 ml |
| RNase Free Water | 15 ml | 30 ml | 120 ml |
Storage and Stability
Proteinase K, MagPure Particles N and MagBind Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stablefor at least 18 months under these conditions.
Experiment Data
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
Description
FluoroDye™ DNA Fluorescent Loading Dye is a ready-to-use 6X DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye™ Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye™ DNA Fluorescent Loading Dye allows the user to immediately visualize electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye™ DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye™ emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.
Features:
Composition
FluoroDye™ DNA Fluorescent Loading Dye is stored in 6X concentration in 60% glycerol and buffered with Tris-HCl and EDTA, containing Bromophenol blue, Xylene cyanol FF and Orange G as tracking dyes.
Storage
Protected from light
-20°C for 24 months
FluoroDye™ DNA Fluorescent Loading Dye is a ready-to-use 6X DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye™ Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye™ DNA Fluorescent Loading Dye allows the user to immediately visualize electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye™ DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye™ emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.
