Bis-propargyl-PEG2

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• 10 discrete bands from 50 bp to 500 bp
• Higher intensity reference band at 250 bp

The 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at 250 bp. This Ladder is ideal for quick sizing of PCR products

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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50 bp DNA Ladder (Cat# 13525) – 100 loads

Ladder Properties:
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	500	79
2	450	67
3	400	59
4	350	55
5	300	49
6	250	76
7	200	33
8	150	22
9	100	31
10	50	29

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Overview

• Purify amplified DNA ranging from 100 bp -15,000 bp in size
• Fast and efficient spin column format 
• Available in a 50 prep size and a 250 prep size
• Also available in 96 well format
   

This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

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Kit Specifications – Spin Column
Column Binding Capacity	10 μg
Size of DNA Purified	100 – 15,000 bp
Average Recovery	> 90%
Average Primer Removal	> 90%
Minimum Elution Volume	30 μL
Time to Complete 10 Purifications	15 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 14400 (50 preps)	Cat. 45700 (250 preps)	Cat. 24800 (192 preps)
Binding Buffer C	30 mL	5 x 30 mL	3 x 30 mL
Wash Solution A	12 mL	2 x 20 mL	2 x 38 mL
Elution Buffer B	8 mL	2 x 30 mL	2 x 15 mL
Spin Columns	50	250	–
Collection Tubes	50	250	–
96-Well Plate	–	–	2
Adhesive Tape	–	–	4
96-Well Collection Plate	–	–	2
Elution Tubes (1.7 mL)	50	250	–
96-Well Elution Plate	–	–	2
Product Insert	1	1	2

Description

The Respiratory Syncytial Virus Rapid Antigen Assay is a lateral flow chromatographic immunoassay for the qualitative detection of RSV. It is suitable for the auxiliary detection of Respiratory Syncytial virus infection. 

For Research Use Only. Not for use in Diagnostic Procedures.

Format: 25 tests per kit
Swab Collection Method: Nasopharyngeal, Anterior Nasal, Oropharyngeal
Results: 20 minutes