Introduction

The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 70μg plasmid DNA from 5-15ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR, cloning, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	High copy plasmid: 1-5ml culture mediumLow copy plasmid : 5-10ml culture medium
Yield	20-80µg
Elution volume	≥75μl
Time per run	Complete 1-24 samples in 30 minutes
Liquid carrying volume per column	800µl
Binding yield of column	70µg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast – it takes only 2 minutes to obtain supernatant by optimized solution (10 minutes for other brands)
• High yield – up to 70µg plasmid can be binded in one column

Kit Contents

Contents	P100202	P100203
Purification Times	50 Preps	250 Preps
RNase A	5 mg	20 mg
Buffer P1	30 ml	140 ml
Buffer P2	30 ml	140 ml
Buffer P3	40 ml	200 ml
Buffer PW1	30 ml	140 ml
Buffer PW2*	12 ml	50 ml
Elution Buffer	15 ml	30 ml
HiPure DNA Mini Columns III	50	250 ml
2 ml Collection Tubes	50	250 ml

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

Name	CAT NO	Features	Sampleamount	Elutionvolume	Yield
HiPure Plasmid Mini Kit	P1001	Classic, Regular application	1-5ml LB	30-50μl	5–35µg/5ml
HiPure Plasmid Mini Kit II	P1002	Classic, Regular application	5-15ml LB	75-100μl	20-80µg/15ml
HiPure Plasmid Plus 96 Kit	P1006	Classic, Regular application	1-1.5ml LB	70-150μl	1-15μg/1ml
HiPure Fastfilter Plasmid Midi Kit	P1013	Fast	30-50ml LB	0.3-0.8ml	50–250µg/50ml
HiPure Fastfilter Plasmid Maxi Kit	P1014	Fast	100-200ml LB	0.5-1.5ml	0.4–1mg/200ml
Low endotoxin/Endotoxin-free Plasmid
HiPure Plasmid EF Maxi Kit	P1156	Classic method, General for all kinds of vectors	100-200ml LB	≥0.7ml	200–1500µg
HiPure Plasmid EF Mini Kit	P1154	Recommend for low copy vector, Thoroughly remove RNA	5-15ml LB	≥60μl	10–80µg
HiPure Plasmid EF 96 Kit	P1157	Recommend for low copy vector, Thoroughly remove RNA	1-5ml LBx96	≥75μl	30µg
HiPure Plasmid EF Midi Kit	P1231	Recommend for High copy vector, Low elution volume, High concentration	25-50ml LB	≥100μl	10-500μg
HiPure Plasmid EF Mega Kit	P1116	Recommend for High copy vector(>3μg/ml)	500ml LB	≥2ml	1-10mg

For any technical problems or customized products, please contact us.

F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here

The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Overview

Low strength adhesive film that is transparent, peelable and suitable for short term storage.

• This transparent polyester-based film has a low strength adhesive
• It is designed as a low-cost sealing option, and useful for temporary storage and as a cover for applications such as centrifugation
• End tabs allow for easy application and removal
• This seal is removable without leaving residue on the plate
• For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer

Low strength adhesive film that is transparent, peelable and suitable for short term storage.

TD5A Low Speed Centrifuge

Features:

1. Microprocessor control, digital display, touch panel, parameters in running can be edited and change to display other running parameter and RCF.

2. Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running.

3. Automatic lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.

4. Adapters are available by experiment requirements.                          

5. 3 tiers protection steel cover, safe and reliable.

6. TUV CE EMC certificate.

TD5A Technical Parameter：

Max. Speed	5000rpm
Max. RCF	4730×g
Max. Capacity	4×300ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 58
Temperature	Normal
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature   Accuracy	/
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (W x D x Hmm)	620×450×355mm
Net Weight(Kg)	52KG
Certificates	CE,ISO & Calibration report are available

Matched Rotor for TD5A

Order NO.	Rotor Type	Max speed(r/min)	Volume (ml)	Max RCF(*g)
5A-1	Swing rotor (round cup)	4000	4×300ml	3180
5A-2	Swing rotor (square cup)	4000	4×300ml	3150
5A-3	Swing rotor	4000	4×24×7ml	3150
		4000	4×24×5ml	2750
		4000	4×18×10ml	3140
5A-4	Microplate rotor	4000	2×4×96 well	2910
5A-5	Microplate rotor	4000	2×4×48 well	2300
5A-6	Swing rotor	5000	4×1×50ml	4730
		5000	4×1×100ml	4730
5A-7	Swing rotor	4000	4×2×50ml	3200
		4000	4×2×100ml	3020
		4000	4×4×15ml	3200
		4000	4×6×15ml	3200
		4000	4×8×15ml	3200
		4000	4×12×5ml vacuum tube	2480
		4000	4×12×7ml vacuum tube	2760
		4000	4×12×10ml vacuum tube	2790
		4000	4×8×10/7/5ml vacuum tube	2790
		4000	4×6×10/7/5ml vacuum tube	2790
		4000	4×4×10/7/5ml vacuum tube	2790
5A-8	Fixed Rotor	5000	6×15ml	2540
5A-9	Fixed Rotor	5000	12×15ml	3080
5A-10	Fixed Rotor	5000	24×15ml	3500
5A-11	Fixed Rotor	5000	30×15ml	3830
5A-12	Fixed Rotor	5000	4×50ml	2520
5A-13	Fixed Rotor	5000	6×50ml	2850
5A-14	Fixed Rotor	5000	12×50ml	3860
5A-15	Fixed Rotor	4000	24×50ml	2970
5A-16	Fixed Rotor	5000	4×100ml	2630
5A-17	Fixed Rotor	5000	6×100ml	3130
5A-18	Fixed Rotor	4000	12×100ml	2970

TD5A centrifuge Max Capacity 4x300ml swing out rotor, max speed 5000rpm, can hold 96 vacuum tubes