Bis-propargyl-PEG7

The RNA Seq Library Prep Kit was developed for construction of high quality NGS  libraries for next generation sequencing (illumina and MGI Platforms). RNA Sequencing is a very powerful tool to analyze transcriptome such as gene expression and transcription regulation, splicing characterization, mutation and variation detection etc. The kit needs purified RNA (example: rRNA depleted RNA or polyA mRNA) as input for library construction. Library multiplexing is possible with different types of indexes.

RNA Seq Library Prep Kit Workflow

Three index types are available for the illumina platform kits:

Non-index (illumina Cat.# 30055): Libraries do not have index.

Index (illumina Cat.# 30056): A unique barcode sequence with 6 bases has been included in each of the index primers. RNA Sequencing library multiplexing is possible with up to 48 samples. Index information can be downloaded here.

Unique dual index (illumina Cat.# 30057): RNA Sequencing library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. the unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34112).

Features

• • • Quick protocol
      • Libraries will be ready in 2 hours
      • Hands-on time is only ~10 minutes
    • Guaranteed quality
      • High yield
      • Uniform coverage of transcripts
    • Simple workflow
    • Input purified RNA amount: From 3 ng to 100 ng

Comparison of the protocol time: BioDynami RNA Seq Library Prep Kit vs other vendors’ kits. Hands-on time and walk-away time were indicated.

Library size and distribution of BioDynami kit, 20 ng and 3 ng of polyA mRNA as input.

Comparison of library yield and duplication rate under the same condition: 20 ng and 3 ng of polyA mRNA were used as input. PCR cycle numbers were indicated.

Comparison of coverage of transcripts: 20 ng of polyA mRNA were used as input. BioDynami kit has more uniform coverage

Overview

• Ensure optimal results for sensitive applications like NGS
• Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
• Purification and enrichment of intact cell culture media exosomes for functional studies
• No phenol extractions, Proteinase K treatment, nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes required
• No precipitation reagents nor overnight incubation required
• Pure exosomes are purified and are free-from any other RNA-binding proteins
• Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allows the user to elute into flexible elution volumes ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, Sequencing based applications, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

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Sample Type	Cell-Free Cell Culture Media
Sample Volume Range	5 ml to 10 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Description

Specifications

Clone	IHC672
Source	Mouse Monoclonal
Positive Control	Testes, Melanoma, Xp11.2 Translocation Renal Cell Carcinoma
Dilution Range	1:200

Transcription Factor E3 (TFE3) is a transcription factor that binds to the MUE3-type E-box sequences involved in TGF-β signalling. Anti-TFE3 staining is the most sensitive and specific indicator of Xp11 translocation renal cell carcinomas. Since alveolar soft part sarcoma (ASPS) is characterized by a specific chromosomal rearrangement resulting in a chimeric transcription factor (ASPSCR1-TFE3), this TFE3 IVD antibody is also a useful diagnostic tool for recognizing ASPS.