Bis-propargyl-PEG12

Description 

The DM1100 ExcelBand™ 50 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM1100 is composed of 17 individual DNA fragments: 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100, and 50 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (500 bp and 200 bp) for easier reference. In addition, the low range Orange G tracking dye mimics the migration of a 50 bp dsDNA during electrophoresis is also added for real time monitoring.

Features

• Sharp bands
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

50 ~ 1,500 bp 

Concentration 

54 µg/ 500 µl  

Recommended loading volume 

5 µl/ well

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months 

The DM1100 ExcelBand™ 50 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM1100 is composed of 17 individual DNA fragments: 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100, and 50 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (500 bp and 200 bp) for easier reference. In addition, the low range Orange G tracking dye mimics the migration of a 50 bp dsDNA during electrophoresis is also added for real time monitoring.

Overview

• Fast, reproducible and easy processing of samples
• High yield and high quality genomic DNA with no RNA or protein contamination
• DNA ready for any application including PCR, qPCR, genotyping and more

Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.

Cells and Tissue DNA Isolation Kit (Spin Column)

Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Micro Kit (Micro)

Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Kit (Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.

Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.

Details

Supporting Data

Figure 1 / 9

Previous

Next

Click for expanded view

Kit Specifications
Maximum Input	20 mg of animal tissue Up to 150 μL of viral suspension 3 x 106 cells
Column Binding Capacity	> 50 μg
Average Yield	8 μg (from 1 x 106 HeLa Cells) 10 μg (from 10 mg kidney)
Elution Volume	50 – 200 μL
Analyte Purified	Genomic DNA, mitochondrial DNA, viral DNA
Format	Spin Column
Time to Complete 10 Purifications	30 min (cells) and 90 min (tissue)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 53100 (50 preps)	Cat. 57300 (50 preps)	Cat. 59100 (50 preps)	Cat. 62500 (192 preps)
Lysis Buffer B	20 mL	20 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	18 mL	18 mL	55 mL
Wash Solution A	18 mL	18 mL	–	–
Elution Buffer B	30 mL	8 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K	1.2 mL	1.2 mL	1.2 mL	–
Proteinase K in Sotrage Buffer	–	–	–	4 mL
Magnetic Bead Suspension	–	–	2 x 1.1 mL	8.5 mL
96-Well Plate	–	–	–	2
Spin Columns	50	–	–	–
Micro Spin Columns	–	50	–	–
Collection Tubes	50	50	–	–
Elution Tubes (1.7 mL)	50	50	50	–
96-Well Elution Plate	–	–	–	2
Adhesive Tape	–	–	–	2
Product Insert	1	1	1	1

DBCO-PEG2-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG2-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.