Bis-propargyl-PEG14 is a homobifunctional crosslinker that can participate in Click Chemistry to yield a stable triazole linkage with azide; copper is needed as a catalyst. With PEG chain embedded in the molecule, the hydrophilicity is greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG14 is a homobifunctional crosslinker that can participate in Click Chemistry to yield a stable triazole linkage with azide; copper is needed as a catalyst. With PEG chain embedded in the molecule, the hydrophilicity is greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Biotin-PEG4-C1-alkyne is azide reactive biotinylation reagent, PEG4 arm increase aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Biotin-PEG4-C1-alkyne is azide reactive biotinylation reagent, PEG4 arm increase aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
Overview
This kit provides a fast and simple procedure for the isolation of total proteins from tissue, bacteria, yeast or mammalian cells without the use of SDS, Triton® X-100 and other detergents. Detergents are extensively used to prepare protein samples; however, these detergents have undesirable effects on downstream analysis. These effects include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization.
The Detergent-Free Total Protein Isolation Kit maintains high protein recovery and yields proteins that are 100% detergent-free. Purification is based on using Norgen’s proprietary resin together with Lysis Solution, followed by protein filtration using a filter column (provided). The purified proteins can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, NMR spectroscopy and more.
Each Lysis Tube is able to process up to 50 mg of tissue, 1010 bacterial cells, 109 yeast cells or 107 mammalian cells. Preparation time for 12 samples is less than 10 minutes.
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| Kit Specifications | |
| Maximum Amount Of Starting Material: Tissues Animal Cells Bacteria Yeast | 50 mg 1 x 107 cells 1 x 1010 cells 1 x 109 cells |
| Time to Process 12 Samples | Less than 10 minutes |
Storage Conditions and Product Stability
The Lysis Solution should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 30300 (50 preps) |
|---|---|
| Lysis Solution | 4 mL |
| Lysis Tubes | 25 |
| Filter Columns | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
