t-Boc-aminooxy-PEG1-propargyl is a click chemistry PEG linker with a t-Boc-aminooxy group. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The t-Boc aminooxy group can be deprotected under mild acidic conditions and then can react with an aldehyde or ketone group to form a stable oxime linkage. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
t-Boc-aminooxy-PEG1-propargyl is a click chemistry PEG linker with a t-Boc-aminooxy group. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The t-Boc aminooxy group can be deprotected under mild acidic conditions and then can react with an aldehyde or ketone group to form a stable oxime linkage. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
HCM114 Bile Esculin Azide Agar
Product Info
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Product Info
Introduction
Intended Use
For the selective separation and enumeration of enterococci in food and water.
Principle and Interpretation
Tryptone and peptone are the sources of nitrogen and essential growth factors. Yeast extract acts as well nitrogenous compounds and additionally the vitamin B12 complex. Sodium azide acts largely inhibits the growth of gram-negative bacteria while sparing enterococci, staphylococci and streptococci. Ox bile inhibits most gram positives but not enterococci. Enterococci hydrolyse esculin to esculetin and dextrose, which reacts with ferric citrate producing a brownish black precipitate around the colonies. Tolerance to bile and the ability to hydrolyze esculin is the traditional and reliable test for the identification of enterococci. (4). Sodium chloride maintains the osmotic balance of the medium and Agar is the solidifying agent.
Formulation
Ingredients
/liter
Tryptone
17.0g
Ox bile
10.0g
Yeast extract
5.0g
Sodium chloride
5.0g
Peptone
3.0g
aesculin
1.0g
Ferric ammonium citrate
0.5g
Sodium azide
0.15g
Agar
15.0g
pH 7.1±0.1 at 25°C
Preparation
Weigh 56.6g of dry powder of this product, add 1 L of distilled water or deionized water, stir, heat and boil until completely dissolved, and sterilize at 121℃ for 15 min.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 20-24hours.
Quality control strains
Growth
Colony color
Enterococcus faecalis ATCC29212
PR≥0.7
Brown-black halo
Escherichia coli ATCC25922
inhibited
Absence of brown-black halo
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the selective separation and enumeration of enterococci in food and water. Principle and Interpretation Tryptone and peptone are the sources of nitrogen and essential grow……
Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
Can process challenging samples such as mastitic milk
Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
Fast and efficient spin-column format
This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples. It can also be used to process challenging milk samples such as Mastitic milk for example. The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk. Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.
*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.
Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.
Q-PAGE™ TGN Precast Gels are available in gradient (4 to 15%) and fixed (10%) concentrations of polyacrylamide in 12- and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP4XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP5XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
User-friendly gel cassette:
Numbered and framed wells for sample loading
Labeled warning sign and green tape as reminder
Enhanced gel performance:
Enhanced gel electrophoresis speed
Better band separation, equivalent to commercially 4-20% TG gels
Stable for shipping at ambient temperature
Easy compatibility:
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis.
Keep Q-PAGE™ Precast Gels flat during storage.
Document
Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.
