t-Boc-aminooxy-PEG2-propargyl is a click chemistry crosslinker. The propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-aminooxy-PEG2-propargyl is a click chemistry crosslinker. The propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s Low Abundance DNA Quantification Kit offers a PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range. The kit consists of a specially designed primer mix, that is used in conjunction with the provided 2x PCR Master Mix, to amplify human DNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown DNA is accurately quantified by using a standard curve constructed from the provided DNA Standard.
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Storage Conditions
Upon receipt, store Norgen’s Low Abundance DNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycle. If needed, prepare smaller working aliquots and store at -20°C or lower.
| Component | Cat. 57200 (48 reactions) |
|---|---|
| 2X PCR Master Mix | 1 mL |
| DNA Quantification Primer Set Mix | 200 µL |
| Quantified DNA Standard | 5 standards, each 100 µL |
| Nuclease-Free Water | 1.25 mL |
| Product Insert | 1 |
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from bacteria culture |
| Applications | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Bacteria culture |
| Sample amount | Bacteria: <10^9 |
| Elution volume | ≥30μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Kit Contents
| Contents | R418101 | R418102 | R418103 |
| Purification Times | 10 Preps | 50 Preps | 250 Preps |
| gDNA Filter Mini Columns | 10 | 50 | 250 |
| HiPure RNA Mini Columns | 10 | 50 | 250 |
| 2ml Collection Tubes | 20 | 100 | 500 |
| Glass Beads (0.1-0.6mm) | 10 g | 30 g | 150 g |
| Plastic spoon | 2 | 4 | 10 |
| Lysozyme | 20 mg | 90 mg | 400 mg |
| Protease Dissolve Buffer | 1.8 ml | 1.8 ml | 10 ml |
| Buffer TE | 1.8 ml | 1.8 ml | 5 ml |
| Buffer STL | 5 ml | 20 ml | 90 ml |
| Buffer RLC | 10 ml | 30 ml | 150 ml |
| Buffer RW1 | 10 ml | 50 ml | 250 ml |
| Buffer RW2* | 5 ml | 20 ml | 2 x 50 ml |
| RNase Free Water | 1.8 ml | 10 ml | 30 ml |
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Due to the lack of antibacterial agents, RNase Free Water may be contaminated by bacterial or fungal when placed or operated at room temperature. It is recommended to pack and store at 2-8°C to reduce contamination.
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA（miRNA）from tissue, cell using two columns and DNase plus reagent |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Products | RNA, miRNA |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Clinical tissues, cells, lymphocytes |
| Sample amount | Tissue: <20 mgCells: <5 x 106 |
| Yield | 2-50μg |
| Elution volume | ≥30μl |
| Time per run | ≤25 minutes |
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.
Advantages
Kit Contents
| Contents | IVD4121 |
| Purification Times | 50 Preps |
| HiPure DNA Mini Column Ⅱ | 50 |
| HiPure RNA Mini Columns | 50 |
| 2ml Collection Tubes | 150 |
| Proteinase K | 24 mg |
| Protease Dissolve Buffer | 1.8 ml |
| DNase I | 600 μl |
| DNase Buffer | 6 ml |
| Buffer RTL | 40 ml |
| RNA Digestion Buffer | 15 ml |
| Buffer RWC* | 20 ml |
| Buffer RW2* | 20 ml |
| Nuclease Free Water | 10 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
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Tel : 081-875-1869 , 02-328-7179
Email : [email protected]
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