Bacterial DNA Extraction Kit (Magnetic Beads)

The Bacterial DNA Extraction Kit (Magnetic Beads) was developed for the bacterial genomic DNA extraction from bacterial cultures directly using magnetic beads from a wide variety of gram-negative and gram-positive bacterial species, as well as yeast and other fungi. With our proprietary magnetic beads technology, the kit eliminates the tedious centrifuge steps for columns. The kit provides a reliable and simple approach for high-quality bacterial DNA isolation with fast magnetic response time and high binding capacity.

Bacteria are the most diverse and abundant small single-celled organisms and are vital to the planet’s ecosystems. Some bacterial species are pathogens that can cause a variety of diseases to humans and animals. Besides their ecological and biomedical importance, bacteria are also used in biotech and pharmaceutical applications such as production of enzymes, DNA preparation, biofuels, food research, and chemical production.

Bacterial cells are grown to log-phase and the culture is lysed directly with a lysis buffer, then mixed with beads to bind genomic DNA. After wash steps, genomic DNA is eluted in Low TE or TE Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities. Bacterial DNA extracted using the kit is suitable for downstream applications such as qPCR, PCR, DNA sequencing, Southern Blotting, molecular cloning, DNA hybridization, restriction enzymatic digestion, and Next-generation Sequencing (NGS) etc.

Features

• 100% centrifuge-free
  • Bacterial cultures can be used directly without centrifuge to pellet the bacteria
• Simple magnetic beads method
  • No centrifuge needed
  • No column needed
  • No vacuum needed

Purified genomic DNA from various bacteria were isolated with the Bacterial DNA Extraction Kit. A portion of the extracted genomic DNA was loaded on a 1% agarose gel. DNA ladder: BioDynami 1 kb Plus DNA Ladder (Cat.# 10005L).

Overview

• The 96 Extraction Tube Seal provides a robust, simple solution for sealing & disposing of all 96 extraction tubes post-run on the BD Viper™ System.
• The seal has been fully tested within Laboratories and has shown to be easy-to-use and enables the one-step removal & disposal of the sealed extraction tubes from the rack.

A robust, simple solution for sealing & disposing of all 96 extraction tubes

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings 150 reactions