Introduction

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments >100bp from agarose gel(<0.2g), purification of DNA from PCR or enzymatic reaction solution solution
Applications	PCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification method	Micro spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Agarose gel, PCR products, enzyme products
Sample amount	Agarose gel: ≤200mgDNA products: ≤300µl (≤5µg)
Recovery	100-5000bp：85%, 5-10kb：60-70%
Elution volume	≥10μl
Time per run	≤30 minutes（1-24 samples）
Liquid carrying volume per column	800µl
Binding yield of column	20µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

• High recovery efficiency – up to 80% DNA recovery
• Low elution volume – elution volume can be as low as 10μl
• General – recover DNA fromgel or enzyme-driven reaction solutions such as PCR
• Fast – isolation can be completed in 10-15 minutes by column gel method

Kit Contents

Contents	D211002	D211003
Purification Times	100 Preps	250 Preps
Buffer GDP	60 ml	125 ml
Buffer DW1	40 ml	90 ml
Buffer DW2	20 ml	50 ml
Elution Buffer	20 ml	30 ml
HiPure DNA Micro Columns	100	250
2 ml Collection Tubes	100	250

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.

DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.

Perfect for:

• End-point gel electrophoresis DNA detection
• down-stream applications (e.g. sub-cloning)
• facilitating use of different RPA reaction volumes, or variation of component ratios.

Product code: TALQBAS01

DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.

• Format: 96-well microtiter plate (12 test strips of 8 wells)
• Standards: 0 | 0.15 | 0.4 | 1 | 2 | 5 ppb
• Incubation Time: 75 Minutes
• Compatible for use with US EPA Method 546

Description

Microcystin are a class of hepatotoxins produced by blue-green algae such as Microcystis aeruginosa.  Microcystin-LR is the most common of the over 50 different congeners. Cyanobacteria can produce microcystin in large quantities during an algae bloom which then pose a major threat.

This kit can be used for Congener-Independent quantitative test of Microcystin in liquid samples such as drinking, ambient and waste water and algal cultures.

Additional Quality Control, Calibration Verification, and Spiking Solution materials can be obtained separately in optional Supplemental Pack for Method 546 (catalog # EL2024-Q).

Microcystin-LR

• Informational sign postings about HABs at recreational waters: < 6 μg/L
• Recreational public health advisory: 6 μg/L
• Elevated recreational public health advisory (e.g. no contact): 20 μg/L

EPA 10-day drinking water health advisories:

• Do Not Drink – 0.3 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions.
• Do Not Drink – 1.6 μg/L for school-age children to adults.
• Do not Use – 20 μg/L

Format: 96-well microtiter plate (12 test strips of 8 wells)
Standards: 0 | 0.15 | 0.4 | 1 | 2 | 5 ppb
Incubation Time: 75 Minutes
Compatible for use with US EPA Method 546