Disulfide Biotin alkyne is an azide-activated cleavable biotin probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. This reagent contains a biotin moiety linked to an alkyne group through a spacer arm containing a cleavable disulfide linker. Under reducing conditions (50 mM dithiothreitol, 10 mM 2-mercaptoethanol or 1% sodium borohydride), the disulfide bonds are cleaved, releasing the biotin tag and any avidin conjugate bound to it.
Disulfide Biotin alkyne is an azide-activated cleavable biotin probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. This reagent contains a biotin moiety linked to an alkyne group through a spacer arm containing a cleavable disulfide linker. Under reducing conditions (50 mM dithiothreitol, 10 mM 2-mercaptoethanol or 1% sodium borohydride), the disulfide bonds are cleaved, releasing the biotin tag and any avidin conjugate bound to it.
Bis-PEG23-endo-BCN is a click chemistry reagent, The BCN group enable copper free click chemitry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-PEG23-endo-BCN is a click chemistry reagent, The BCN group enable copper free click chemitry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Description
The PM2500 ExcelBand™ 3-color Regular Range Protein Marker is a ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). PM2500 ExcelBand™ 3-color Regular Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Quality Control
Under suggested conditions, PM2500 ExcelBand™ 3-color Regular Range Protein Marker resolves 10 major bands in 15% SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months
-20°C for long term storage
The PM2500 ExcelBand™ 3-color Regular Range Protein Marker is a ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). PM2500 ExcelBand™ 3-color Regular Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Attogene’s Microcystin Test Kit (Rapid – Recreational Water) can be used to detect microcystins in liquid samples; highly sensitive, rapid, robust screening kit for algae toxins microcystins and nodularins.
The most frequently reported cyanobacterial toxins are the hepatotoxic microcystins (MCs). MCs are peptides with a molecular weight ranging from 900 to 1,100 Da. They consist of seven amino acids of which the two terminal amino acids of the linear peptide are condensed to form a cyclic compound.
A tiered notification system which takes different actions based on different numeric thresholds for microcytin-LR concentrations in recreational waters has been developed. This is guidance that allows states to take various actions—such as posting information about harmful algal blooms (HABs), issuing a recreational public health advisory, or temporarily closing recreational waters through a no contact advisory—depending on the severity of the bloom event.
Microcystin-LR
Informational sign postings about HABs at recreational waters: < 6 μg/L
Recreational public health advisory: 6 μg/L
Elevated recreational public health advisory (e.g. no contact): 20 μg/L
Screening of Microcystins in water samples at 6 ppb or lower
Format: 10 tests (5 tests/5 control)
Water Sample Bottles
Negative Control
1mL Syringe
Sample Dilution Buffer also sold separately
Sample Filters
