Biotin-PEG2-alkyne is a short PEG linker featuring a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azides.

Biotin-PEG2-alkyne is a short PEG linker featuring a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azides.

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from bacterial culture
Applications	PCR, southern blot and enzyme digestion, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Gram negative or positive bacteria
Sample amount	Bacterial culture: 0.5-1.5ml
Elution volume	≥60μl
Time per run	≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• Fast – several samples can be extracted in 60 minutes (after digestion)
• High purity – unique adsorbent can completely remove inhibitory factors
• High recovery – DNA can be recovered at the level of PG

Kit Contents

Contents	D513101	D513102	D513103
Purification Times	48 Preps	96 Preps	480 Preps
MagPure Particles	1.2 ml	2.5 ml	11 ml
Lysozyme	60 mg	120 mg	600 mg
Proteinase K	24 mg	50 mg	220 mg
Protease Dissolve Buffer	3 ml	6 ml	30 ml
Buffer SDS	1.5 ml	3 ml	15 ml
Buffer MLA	30 ml	60 ml	300 ml
Buffer GW1*	13 ml	44 ml	110 ml
Buffer TE	30 ml	40 ml	200 ml

Storage and Stability

MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.

Heat Sealing Time:		0.5~2s			Heat Sealing Tube Outer Diameter:		2~6mm
Indicator Light Colors:		Red/Blue			RF Frequency:		40.68MHz
Grounding Protection:		Class l			Spacing Adjustment		65,70,75,80,85,90,95,100mm
Power Consumption:		250W during operation,10Win standby			Power Supply:		100V~120V/220V~240V AC
Weight:		4.5kg			Dimensions:		336×65×173mm
Operating Temperature:		0~40℃			Storage Temperature:		-20~70℃

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