Disulfide Biotin alkyne is an azide-activated cleavable biotin probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. This reagent contains a biotin moiety linked to an alkyne group through a spacer arm containing a cleavable disulfide linker. Under reducing conditions (50 mM dithiothreitol, 10 mM 2-mercaptoethanol or 1% sodium borohydride), the disulfide bonds are cleaved, releasing the biotin tag and any avidin conjugate bound to it.
Disulfide Biotin alkyne is an azide-activated cleavable biotin probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. This reagent contains a biotin moiety linked to an alkyne group through a spacer arm containing a cleavable disulfide linker. Under reducing conditions (50 mM dithiothreitol, 10 mM 2-mercaptoethanol or 1% sodium borohydride), the disulfide bonds are cleaved, releasing the biotin tag and any avidin conjugate bound to it.
Cell membrane preparations containing nicotinic AChRs that are ligand-gated ion channels that form pores in cells plasma membranes, mediating fast signal transmission at synapses. Nicotinic AChRs are involved in a wide range of physiological processes, and can be either neuronal or muscle-type. The membrane preparations we have developed are suitable for receptor binding assays in which muscle type nicotinic AChRs are needed. The membranes are tested in several functional binding assays and quality testing criteria to meet binding specifications.
Cell Membrane Preparation containing nAchR
The Residual DNA Sample Preparation Kit uses chemical lysis and magnetic beads to extract DNA from diverse sample types, including samples that contain high protein and low DNA concentration. The kit extracts residual genomic DNA from products that are produced in cell lines such as CHO, E. coli, E1A, HEK293, Vero, NS0 and Baculovirus. For quantification of residual DNA, we recommend using the resDNASEQ Residual DNA Quantitation kit as described in the resDNASEQ Residual DNA Quantitation kit User Guide. To ensure accurate quantitative results, each sample in triplicate and perform a single PCR reaction for each extraction.
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Only three steps will be need for Sample Preparation，and the total time of Sample preparation is about 30 minites.
The good Recovery rate of Sample Preparation.
All components of the Sample Preparation Kit can be stored at room temperature.
Note: Price not include shipment & duty, contact us to get full quote.
The Residual DNA Sample Preparation Kit uses chemical lysis and magnetic beads to extract DNA from diverse sample types, including samples that contain high protein and low DNA concentration. The kit extracts residual genomic DNA from products that are produced in cell lines. The operation is easier and the higher nucleic acid yield is guaranteed at the same time.
| Clone | IHC654 |
| Source | Mouse Monoclonal |
| Positive Control | Prostate, Prostate Carcinoma |
| Dilution Range | 1:200 |
Prostate-Specific Antigen (PSA) is a serine protease of the kallikrein family, that is produced by the prostate epithelium and epithelial lining of the periurethral glands. Although considered prostate-specific, PSA has also been detected in breast tissue, breast tumors, endometrium, adrenal neoplasms, and renal cell carcinomas. Anti-PSA can be used for differentiating high-grade prostate adenocarcinoma from high-grade urothelial carcinoma, as well as for determining the prostatic origin of carcinomas in non-prostate tissues. Anti-PSA recognizes primary and metastatic prostatic neoplasms, but not tumors of nonprostatic origin, and can be useful as an aid to confirm prostatic acinar cell origin in primary and metastatic carcinomas.
