m-PEG17-propargyl

Introduction

Usages：
For determination of coliform confirmatory test for multi-tube fermentation.
 
Principle:
Peptone provide carbon and nitrogen sources; lactose as fermentable sugars; ox bile powder and brilliant green inhibit the non-Enterobacteriaceae.
 
Formulation (per liter):
Peptone: 10g
Lactose: 10g
Ox bile powder: 20g
Brilliant green: 0.0133g
Final pH7.2 ± 0.2
 
How to use:
1.Suspend 40g of the product, add 1 L of distilled water, stirring heated to boiling until completely dissolved, packed in a test tube with a small inverted tube, autoclave at 121 ℃ for 15min,  cool to room temperature and set aside.
2.Sample handling and dilution. Slightly.
3.Transplanted LST broth tube to brilliant green lactose bile broth tube by using a inoculating loop.
4.the tube was placed in an incubator culture at 36 ± 1 ℃ for 24h.
5.Observe the results. Press production trachea number, check table report MPN MPN value per gram (mL) sample of coliform bacteria.
 
Quality control:
Quality control strains were inoculated and culuture at 36 ± 1 ℃ for 24h ,results are as follows:
Bacterial Name                 Bacterial No.          Growth Status       Gassing
Escherichia coli                ATCC25922               good              +
 
Salmonella typhimurium        CMCC (B) 50115            good             —
 
Staphylococcus aureus          ATCC6538                inhibited          —
 
 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
 
Specifications: 250g/bottle

250g

Description

Attogene Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a lateral flow dipstick assay for detection of  DNA and RNA products.

Formats (strep gold conjugate pad):

Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification.  Test line: anti-FITC/FAM, Control Line: Biotin

Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FITC/FAM and Dig labelled primers during amplification.: Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 Biotin.

Inquire about custom configurations: sales@attogene.com

Kit Components 

• 50 -4.5mm Lateral Flow Dipsticks
• 10 mL Sample assay running buffer

Features & Benefits

• Can be used for development of a lateral flow assay for detection of a variety of different molecules such as amplified DNA products from PCR, LAMP and RPA reactions.
• No need to stripe capture antibodies
• No expensive equipment required
• Cost-effective way to screen for further downstream lateral flow assay development.

50 Lateral Flow Dipsticks (4.5mm)
10 mL Sample assay running buffer
96 well plate
Controls

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request