endo-BCN-pentanoic acid is a click chemistry reagent containing a BCN group and a terminal carboxylic acid. The BCN group can react with azide-tagged compounds or biomolecules. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-pentanoic acid is a click chemistry reagent containing a BCN group and a terminal carboxylic acid. The BCN group can react with azide-tagged compounds or biomolecules. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Permagen’s XLE PCR-96 low volume bar magnet plate for PCR applications features an individual bar magnet for each row of wells, making it the perfect solution for hand pipetting as all of the pelleted beads will be to the same side in each row. With its SBS footprint and integrated cushion base, scaling up to the robot is a snap. Available in kit form as well, which includes our X39602 top plate for 0.2 mL tubes & strips
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
A012424 (magnet plate only)
A012424KT (Includes top plate kit for 0.2 mL strips and tubes)
*only recommended for manual use as semi-, and non-skirted plates will not locate correctly into the nest but can be positioned against the magnet
Minimum – 5 µL
Maximum – 120 mL
Permagen’s XLE PCR-96 low volume bar magnet plate for PCR applications features an individual bar magnet for each row of wells, making it the perfect solution for hand pipetting as all of the pelleted beads will be to the same side in each row. With its SBS footprint and integrated cushion base, scaling up to the robot is a snap. Available in kit form as well, which includes our X39602 top plate for 0.2 mL tubes & strips
Norgen’s microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix. The kit utilizes Norgen’s microScript Reverse Transcriptase, a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It has reduced RNase H activity and increased thermal stability.
The workflow of Norgen’s microScript microRNA cDNA Synthesis Kit involves a simple, single-tube set-up by the mixing of 2x Reaction Mix, Enzyme Mix and the RNA template. The reaction can then be carried out in a thermocycler. A poly (A) tail is first added to the RNA template, followed by cDNA synthesis using an adapter primer. In addition to the ease-of-use, the single-tube set-up provides superb consistency and sensitivity. The cDNA could be used in a PCR or qPCR amplification using a Universal PCR Reverse Primer and the forward primer that contains the sequence of the microRNA of interest. A single cDNA preparation could be used for PCR amplification of a number of different microRNAs. In addition, the cDNA preparation could be used for PCR or qPCR detection (using gene-specific forward and reverse primers) of mRNA or large RNA if total RNA preparation was the starting template. This could allow for parallel evaluation of expression level of microRNAs and microRNA-targets.
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Storage Conditions and Product Stability
Norgen’s microScript microRNA cDNA Synthesis Kit components should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 54415 (12 rxns) | Cat. 54410 (50 rxns) |
|---|---|---|
| microScript microRNA Enzyme Mix | 12 μL | 50 μL |
| 2x Reaction Mix | 120 μL | 500 μL |
| Universal PCR Reverse Primer | 60 μL | 250 μL |
| Nuclease-Free Water | 1.25 mL | 2 x 1.25 mL |
| Product Insert | 1 | 1 |
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by infection of various bacteria. Streptococcus uberis is a gram-positive bacterium that is known worldwide as an environmental pathogen responsible for a high proportion of cases of mastitis in lactating cows and is also the predominant organism isolated from mammary glands during the non-lactating period. Often it is resistant to treatment and causes persistent high somatic cell counts without clinical mastitis.
Streptococcus uberis TaqMan PCR Kit, 100 reactions
Streptococcus uberis TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 3
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM30850 (100 preps) | Cat. TM30810 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| S. uberis Primer & Probe Mix | 280 μL | 280 μL |
| S. uberis Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
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