Overview

• Detection kits for Listeria monocytogenes
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis.

Listeria monocytogenes have emerged as significant foodborne pathogens that pose a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality rate among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium that could survive high and low temperatures, low pH. It is a rare causative agent of mastitis in cow. However, due to its ability to resist various food processing technologies as well as to grow at low temperature, L. monocytogenes is know to be associated with both raw and pasteurized milk, as well as dairy products such as cheese. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals the most susceptible.

Listeria monocytogenes TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Listeria monocytogenes TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM30450 (100 preps)	Cat. TM30410 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Listeria monocytogenes Primer & Probe Mix	280 μL	280 μL
Listeria monocytogenes Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Introduction

This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.

Details

Kit Contents

Contents	D312402	D312403
Purification Times	50	250
HiPure DNA Mini Columns I	50	250
2ml Collection Tubes	50	250
Buffer BGL	40 ml	180 ml
Buffer GXP	30 ml	150 ml
Buffer GW1*	13 ml	53 ml
Buffer GW2*	20 ml	2 x 50 ml
DTT Powder	235 mg	2 x 235 mg
Proteinase K	48 mg	240 mg
Protease Dissolve Buffer	5 ml	15 ml
Elution Buffer	15 ml	60 ml

Storage and Stability

This product can be stored at room temperature (15~25°C) for 18 months. Proteinase K/DTT dry powder can be transported and stored at room temperature. For long-term storage (>6 months), it is recommended to store at -20~8°C. Dissolved Proteinase K should be stored at -20~8°C. The dissolved DTT should be stored at -20°C.

This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.