endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Features:
1. Programmable microprocessor control system, touch screen indicates rotor No., speed, time, temperature, RCF, rate of speed as well as fault information.
2. Adopts band heater, fast heating speed, temperature uniform, with the function of temperature-control and constant.
3. Centrifugal tube made in special skill, anti-broken when work.
4. Great toque brushless motor, maintenance-free, no powder, fast acceleration and deceleration, low noise, which improve the stability a lot.
5. Electric lid lock, over-speed and over-temperature protection
6.3 phase to keep the best performance.
7. Stainless steel chamber, safe and reliable
TD5B Technical Parameter：
| Max. Speed | 4000rpm |
| Max. RCF | 2498×g |
| Max. Capacity | 4×100ml |
| Time Range | 0~99min |
| Rotor | Swing Out Rotor |
| Noise (dB) | ≤ 58 |
| Temperature | Room temperature:+10~80℃ |
| Acc/Dcc | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Oil tube size | 37×200mm |
| Size (W x D x Hmm) | 685×500×385mm |
| Net Weight(Kg) | 67KG |
Oil test centrifuge， Heating oil test centrifuge, special tube oil test centrifuge
The Kit provides fast purification of high-quality RNA from paxigene Blood RNA Tube using silica-membrane spin columns. RNA purified using the HiPure System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from Paxgene RNA Tubes |
| Applications | RT-PCR, qPCR, Northern hybridization, NGS, nucleic acid protection, in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology, DNA filtration technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Blood in the preservation tube |
| Sample amount | 2.5ml |
| Elution volume | ≥30μl |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100μg |
The Kit is for the purification of total RNA from 2.5 ml human whole blood collected in a PAXgene Blood RNA Tube. Purification begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed and resuspended, and incubated in optimized buffers together with proteinase K. DNA wash The lysate is passed through a DNA Mini column. Ethanolis added to adjust binding conditions, and the lysate is applied to a column.RNA is selectively bound to the silica membrane as contaminants pass through. Remaining contaminants are removed in several efficient wash steps. Between the first and second wash steps, the membrane is treated with DNase I to remove trace amounts of bound DNA. After the wash steps, RNA is eluted in elution buffer and heat-denatured.
Advantages
Kit Contents
| Contents | R416802 | R416803 |
| Purification Times | 10 Preps | 50 Preps |
| HiPure RNA Mini Columns I | 10 | 50 |
| gDNA Filter Mini Columns | 10 | 50 |
| 2ml Collection Tubes | 30 | 150 |
| RNase Free Water | 60 ml | 250 ml |
| Buffer MBR1 | 10 ml | 30 ml |
| Buffer MBR2 | 5 ml | 15 ml |
| Buffer RW1 | 15 ml | 60 ml |
| Buffer RW2* | 6 ml | 20 ml |
| Proteinase K | 12 mg | 50 mg |
| Protease Dissolve Buffer | 1.8 ml | 5 ml |
| DNase I | 120 µl | 600 µl |
| DNase Buffer | 6 ml | 30 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Experiment Data
The Kit provides fast purification of high-quality RNA from paxigene Blood RNA Tube using silica-membrane spin columns. RNA purified using the HiPure System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Attogene has developed the first of its kind and patent pending technology that uses a novel RNA/DNA substrate tagged on the 5′ and 3′ end with a Biotin that is attached to our streptavidin colloidal gold reporter molecules and a test line tag. In the absence of RNases H, the gold tagged RNA/DNA molecule will flow up the lateral flow strip and bind to the test line using an anti tag antibody (INDICATING that NO RNase H is detected). When RNases H is present or added, however, the RNA strand of the RNA/DNA substrate is cleaved, and the Biotin tagged 5′ and 3′ tags are spatially separated in solution cauing the test line to disapear.
This test can be used to rapidly and efficiently detect ribonucleases H activity and is a perfect tool for monitoring unit activity or residual RNAse H activity.
RNase H activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase H activity in enzyme preparations and determination of RNase H unit activity.
