bis-PEG23-endo-BCN

Propargyl-PEG4-(CH2)3-methyl ester consists of a propargyl group and a methyl ester. The propargyl group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions to form a stable triazole linkage. Under strong basic condition, methyl ester can be hydrolyzed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG4-(CH2)3-methyl ester consists of a propargyl group and a methyl ester. The propargyl group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions to form a stable triazole linkage. Under strong basic condition, methyl ester can be hydrolyzed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Detection kits for Candida albicans
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

Candida albicans is an opportunistic yeast and is the most common fungal pathogen found in the human body. C. albicans can be detected in the gastrointestinal tract, mouth, and vagina of approximately half of healthy adults. It is typically a commensal organism and makes up part of the natural human microflora; however it can overgrow and become pathogenic as a result of various conditions. For example, individuals who have taken recent courses of antibiotics, have a weakened immune system or have diabetes have an increased risk of developing a Candida albicans infection. When an overgrowth occurs, this can lead to common infections such as urinary tract infections, genital yeast infections, oral thrush, and mucocutaneous candidiasis. In the more severe cases, when Candida albicans enters the bloodstream or organs, it can lead to loss of sight, blood and bone infections, endocarditis, meningitis or inflammation of the intraabdominal lining.

Details

Supporting Data

Figure 1 / 3

Previous

Next

Click for expanded view

Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM34050 (100 preps)	Cat. TM34010 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Candida albicans Primer & Probe Mix	280 μL	280 μL
Candida albicans Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 4 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The ITS2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the fungal target ITS2 libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XPMagneticBeads(supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The ITS2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the ITS2 region of the fungal DNA. The post-PCR reaction is then cleaned up using AMPure XPbeads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XPbeads. The libraries are then ready for quantification, pooling and sequencing.

Details

Supporting Data

Figure 1 / 3

Previous

Next

Click for expanded view

Minimum amount of starting material:	2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:	4 hours

Storage Conditions and Product Stability
Norgen’s ITS2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components will remain stable for at least 1 year when stored at the specified storage conditions.