DBCO-PEG1-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-PEG1-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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HCM070 Enterobacteriaceae Enrichment Broth Mossel
Product Info
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Product Info
Introduction
Intended Use
For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products.
Principle and Interpretation
Pancreatic digest of gelatin provide protein, vitamins and amino acids; glucose carbon source; disodium hydrogen phosphate and potassium dihydrogen phosphate as a buffer; ox bile and brilliant green as selective antibacterial agent, inhibiting the growth of non-Enterobacteriaceae.
Formulation
Ingredients
/liter
Pancreatic digest of gelatin
10g
Glucose monohydrate
5g
Dehydrated ox bile
20g
Potassium dihydrogen phosphate
2g
Disodium hydrogen phosphate dihydrate
8g
Brilliant green
15mg
pH7.2±0.2 at 25°C
Preparation
Suspend 45g in 1L of distilled water,stir until completely dissolved and dispense 100 mL into test tubes , heat at 100 °C for 30 min in a waterbath or flowing steam.
Quality Control
The following quality control strains were inoculated and cultured at 30-35℃ for 24h-48h. The results are as follows:
Quality control strains
Inoculum (CFU)
Growth
Escherichia coli ATCC8739
< 100
Good growth
Pseudomonas aeruginosa ATCC9027
Staphylococcus aureus ATCC6538
> 100
Inhibited
Storage and Shelf Life
2-30℃,Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for
30 minutes.
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Intended Use For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products. Principle and Interpretation Pancreatic digest of gelatin pro……
N-(t-butyl ester-PEG2)-N-bis(PEG2-propargyl) is a multi-functional PEG linker with two terminal propargyl groups and a t-butyl ester. The propargyl groups enables formation of triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. The t-butyl protected carboxyl group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
N-(t-butyl ester-PEG2)-N-bis(PEG2-propargyl) is a multi-functional PEG linker with two terminal propargyl groups and a t-butyl ester. The propargyl groups enables formation of triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. The t-butyl protected carboxyl group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could simplify your site-specific analysis of such modifications. The 2′-O-Me sensitive DNA polymerase was engineered to catalyse DNA synthesis from both DNA and RNA and to quantify 2′-O-methylation of nucleotides site-specifically from RNA by real-time PCR. For further information refer to the original publication.
Available upon request and for R&D use only – Contact Us
The 2′-O-Me sensitive DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer.
The enzyme can also be used for real-time cycling, when adding a suitable dye.
Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could simplify your site-specific analysis of such modifications. The 2′-O-Me sensitive DNA polymerase was engineered to catalyse DNA synthesis from both DNA and RNA and to quantify 2′-O-methylation of nucleotides site-specifically from RNA by real-time PCR. For further information refer to the original publication.