Sulfo DBCO-PEG3-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain and sulfo group increase water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-PEG3-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain and sulfo group increase water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
TG16E Technical Parameter：
| Max. Speed | 16000rpm |
| Max. RCF | 19040×g |
| Max. Capacity | 6×10ml |
| Time Range | 0~99min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 65 |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 370×290×215mm |
| Net Weight(Kg) | 16KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for TG16E
| Order No | Rotor | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
| 16E-1 | Angle rotor | 14000 | 4×8PCR | 12070 |
| 16E-2 | Angle Rotor | 16000 | 12×1.5/2ml | 17940 |
| 16E-3 | Angle Rotor | 14000 | 24×1.5/2ml | 17950 |
| 16E-4 | Angle Rotor | 16000 | 10×5ml | 17880 |
| 16E-5 | Angle Rotor | 13000 | 6×10ml | 14190 |
| 16E-6 | Angle Rotor | 12000 | 24 pieces capillary vessel | 15800 |
| 16E-7 | Angle Rotor | 16000 | 40×0.2ml | 19040 |
| 16E-8 | Angle Rotor | 13000 | 40×0.5ml | 17210 |
| 16E-9 | Angle Rotor | 13000 | 30×0.5ml | 13900 |
Features:
1. Brushless motor, no pollution, free-maintenance.
2. Microprocessor control, LCD display which indicates the speed, time, RCF in operation, 10 kinds of brake setting, operate simply.
3. Electric lid lock, super speed, imbalance protection. The centrifuge body is made of high quality steel, stainless steel chamber, safe and reliable.
4. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
5. 3 tiers protection steel cover and get the ideal centrifugation result
This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation high pure total DNA from FFPE using high bind beads |
| Applications | PCR and viral DNA detection, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Paraffin embedded tissue samples |
| Sample amount | 1-6 slices of 10-20μm |
| Elution volume | ≥30μl |
| Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
| Contents | D632301D | D632302D |
| Purification Times | 48 Preps | 96 Preps |
| MagPure Particles N | 1.1 ml | 2.5 ml |
| RNase A | 10 mg | 20 mg |
| Proteinase K | 24 mg | 48 mg |
| Protease Dissolve Buffer | 3 ml | 6 ml |
| Buffer DPS | 60 ml | 100 ml |
| Buffer ATL | 15 ml | 30 ml |
| Buffer BST1 | 30 ml | 60 ml |
| Buffer BW1 | 13 ml | 44 ml |
| Elution Buffer | 15 ml | 30 ml |
Storage and Stability
Proteinase K, RNase A and MagPure Particles N should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.
