DBCO-Sulfo-NHS ester

Description

Attogene’s Mercury Lateral Flow Kit can be used for the screening of Mercury in water and food (fish) samples at 10 ppb in a laboratory setting.  Unlike the field-based kit, the lab kit is intended for a more technical end user who will be evaluating samples in a laboratory setting.  The instructions include method for extraction from food samples.

Mercury contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Mercury ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases.   Long-term health consequences of drinking Mercury-contaminated food and water include brain, heart, kidney, lungs  and immune system problems for adults, and the physical and mental development delays in infants and children.  Attogene’s Mercury Lateral Flow test gives results conforming of 10ppb or greater.  Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 5 minutes.

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Applications for Fish and Shellfish possible:

Mercury Levels in Commercial Fish and Shellfish (1990-2012)

Fish with recognized high concentrations of Mercury:

• Tuna, Grouper, Mackerel, Weakfish, Bluefish, Sablefish, Halibut, Bass Chilean, Shark, Marlin, Tilefish,

Screening of Mercury in water samples at 10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 5 Minutes

Description

Attogene Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a lateral flow dipstick assay for detection of  DNA and RNA products.

Formats (strep gold conjugate pad):

Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification.  Test line: anti-FITC/FAM, Control Line: Biotin

Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FITC/FAM and Dig labelled primers during amplification.: Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 Biotin.

Inquire about custom configurations: sales@attogene.com

Kit Components 

• 50 -4.5mm Lateral Flow Dipsticks
• 10 mL Sample assay running buffer

Features & Benefits

• Can be used for development of a lateral flow assay for detection of a variety of different molecules such as amplified DNA products from PCR, LAMP and RPA reactions.
• No need to stripe capture antibodies
• No expensive equipment required
• Cost-effective way to screen for further downstream lateral flow assay development.

50 Lateral Flow Dipsticks (4.5mm)
10 mL Sample assay running buffer
96 well plate
Controls

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments
• Double intensity reference bands at 200 bp and 400 bp

The Norgen MiniSizer 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments with double intensity reference bands at 200 bp and 400 bp. The MiniSizer is ideal for PCR product size confirmation.

Contents:

1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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MiniSizer 50 bp DNA Ladder (Cat# 11200) – 100 loads

Ladder Properties:
• Fourteen discrete bands, ranging from 50 bp to 650 bp
• Higher intensity bands at 200 bp and 400 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	650	61
2	600	46
3	550	36
4	500	30
5	450	35
6	400	85
7	350	27
8	300	25
9	250	16
10	200	54
11	150	33
12	100	21
13	50	17
14	25	14

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.