Genomic DNA Extraction Kit (HMW, Magnetic Beads)

The Genomic DNA Extraction Kit (HMW, Magnetic Beads) provides a reliable and fast process for extracting high molecular weight (HMW) genomic DNA from cells, blood, and tissues using Solid Phase Reversible Immobilization (SPRI) magnetic beads. With our proprietary magnetic beads technology, the kit eliminates the tedious centrifuge steps for columns. The kit provides a reliable and simple approach for high-quality genomic DNA isolation with fast magnetic response time and high binding capacity.

Cat.# 50014 Genomic DNA Extraction Kit for Cells (HMW, Magnetic Beads)
Cat.# 50015 Genomic DNA Extraction Kit for Blood (HMW, Magnetic Beads)
Cat.# 50016 Genomic DNA Extraction Kit for Tissues (HMW, Magnetic Beads)

The extracted HMW genomic DNA size ranges are dependent on the beads resuspension: 50-150 kb by tube tapping and 40-100 kb by tube vortexing. Purified DNA is recovered at high yield and high purity without RNA contamination. The typical purity ratios of A260/A280 are around 1.8-2.0, and A260/A230 are around 2.2-2.5. Purified HMW genomic DNA is suitable for applications such as long-read sequencing, linked-read genome assembly, long range PCR, optical mapping, and other general applications.

Features

• High molecular weight DNA: 50 kb to 150 kb
• High purity
• Simple magnetic beads method
  • No centrifuge needed
  • No column needed
  • No vacuum needed

A portion of the extracted genomic DNA samples were loaded on a PFGE gel with a DNA ladder indicated. Sample A: liver tissue; Sample B: intestine tissue; Sample C: whole blood; Sample D: cultured 293T cells.

Cultured Cell samples

Cultured cells are collected and are resuspended in a buffer and then lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in the Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.

Blood samples

Whole blood is resuspended in the RBC buffer to remove RBC. The remaining leucocytes are lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.

Tissue samples

Tissues are homogenized and lysed, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.

Features: 

1. Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running

2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of  high quality steel, safe and reliable.

3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable.

4. Microprocessor control.

5. Digital display in time, speed and RCF.

6. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

7. 3 tiers protection steel cover, stainless steel chamber, safe and reliable.

 TG20Technical Parameter：

 Matched Rotors for TG20 

Description

Tyrosinase Inhibitor Screening Kit (Colorimetric) provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of tyrosinase inhibitors. Tyrosinase catalyzes the oxidation of tyrosine, producing a chromophore that can be detected at OD = 510 nm. In the presence of kojic Acid, a reversible inhibitor of tyrosinase, the rate of oxidation of the substrate is decreased.

Tyrosinase or polyphenol oxidase is an oxidoreductase that participates in the biosynthesis of melanin, a ubiquitous biological pigment found in hair, eyes, skin, etc. Inhibition of tyrosinase has been a long-time target in the skin health research, cosmetics and agricultural industries because of its role in browning reactions in skin pigmentation and during fruit harvesting and handling.

For rapid, sensitive and accurate screening of potential Tyrosinase inhibitors