DBCO-PEG4-PFP ester is a PFP active ester containing a DBCO group which can undergo copper-free Click Chemistry with azide (N3). The PFP ester moiety is reactive with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. The hydrophilic PEG linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-PFP ester is a PFP active ester containing a DBCO group which can undergo copper-free Click Chemistry with azide (N3). The PFP ester moiety is reactive with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. The hydrophilic PEG linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments >100bp from agarose gel(<0.3g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA |
| Applications | PCR, NGS, chip analysis, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Agarose Gel, PCR products, enzymatic reaction solution |
| Sample amount | Agarose Gel: <0.3g, PCR products: ≤50µl |
| Recovery | 80% |
| Elution volume | ≥30μl |
| Time per run | ≤30 minutes |
The Kit method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments (>100bp) and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
Kit Contents
| Contents | D500101 | D500102 | D500103 |
| Purification Times | 50 Preps | 500 Preps | 5000 Preps |
| Buffer GDP | 30 ml | 250 ml | 3 x 900 ml |
| MagPure Particles | 1.6 ml | 15 ml | 3 x 50 ml |
| Buffer DW1 | 20 ml | 180 ml | 2 x 800 ml |
| Elution Buffer(10mmTris, pH8.5) | 10 ml | 60 ml | 500 ml |
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
t-Boc-N-Amido-PEG7-propargyl is a crosslinking reagent that enables the formation of triazole linkage with azides under the catalyzation of copper. The Boc-protected amine can be deprotected under mild acidic conditions. By introducing PEG chain into the molecule, the hydrophilicity can be greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-N-Amido-PEG7-propargyl is a crosslinking reagent that enables the formation of triazole linkage with azides under the catalyzation of copper. The Boc-protected amine can be deprotected under mild acidic conditions. By introducing PEG chain into the molecule, the hydrophilicity can be greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
