Propargyl-PEG1-acid is a crosslinker with a propargyl group and a carboxylic acid group. The carboxylic acid reacts with primary amine under the activation of HATU or EDC. The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG1-acid is a crosslinker with a propargyl group and a carboxylic acid group. The carboxylic acid reacts with primary amine under the activation of HATU or EDC. The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products.
Pancreatic digest of gelatin provide protein, vitamins and amino acids; glucose carbon source; disodium hydrogen phosphate and potassium dihydrogen phosphate as a buffer; ox bile and brilliant green as selective antibacterial agent, inhibiting the growth of non-Enterobacteriaceae.
| Ingredients | /liter |
| Pancreatic digest of gelatin | 10g |
| Glucose monohydrate | 5g |
| Dehydrated ox bile | 20g |
| Potassium dihydrogen phosphate | 2g |
| Disodium hydrogen phosphate dihydrate | 8g |
| Brilliant green | 15mg |
| pH7.2±0.2 at 25°C | |
Suspend 45g in 1L of distilled water,stir until completely dissolved and dispense 100 mL into test tubes , heat at 100 °C for 30 min in a waterbath or flowing steam.
The following quality control strains were inoculated and cultured at 30-35℃ for 24h-48h. The results are as follows:
| Quality control strains | Inoculum (CFU) | Growth |
| Escherichia coli ATCC8739 | < 100 | Good growth |
| Pseudomonas aeruginosa ATCC9027 | ||
| Staphylococcus aureus ATCC6538 | > 100 | Inhibited |
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for
30 minutes.
Intended Use For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products. Principle and Interpretation Pancreatic digest of gelatin pro……
This kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, bacterial cells, plants, and blood.
Two columns are provided with this kit. The first column captures large RNA, while the small RNA are captured on a second column and are eluted concentrated in 25 µL of nuclease-free water.
The small RNA can be used in various downstream applications relating to miRNA profiling, gene regulation, and functional analysis. The eluted RNA is ready for RT-qPCR, microarrays, and NGS applications.
Background
These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation.
Figure 1 / 4
Click for expanded view
| Kit Specifications | |
| Maximum Column Binding Capacity | Up to 50 μg RNA |
| Maximum Column Loading Volume | 650 μL |
| Minimum Elution Volume | 20 μL |
| Size of RNA Purified | All sizes, including small RNA < 200 nt |
| Time to Complete 10 Purifications | 25 minutes |
| Maximum Amount of Starting Material: Animal Cells Animal Tissues Bacteria Plant Tissues Blood | 3 x 106 cells 5-25 mg 1 x 109 cells 50 mg 100 μL |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 21300 (25 preps) |
|---|---|
| Buffer RL | 40 mL |
| Wash Solution A | 38 mL |
| Elution Solution A | 6 mL |
| Large RNA Removal Column | 25 |
| microRNA Enrichment Column | 25 |
| Collection Tube | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Solid Phase Adsorption Toxin Tracking (SPATT) is a biomimetic in-situ water monitoring tool that falls under an expanding umbrella of passive samplers. It serves to warn researchers of toxin-producing harmful algal bloom (HAB) developments early on. It has been popularized through its affordability, ease of use, and its ability to capture ephemeral events in marine, brackish, and freshwater environments. Its uptake of contaminants has been shown to be more similar than other sampling methods to that of aquatic species like bivalves, mussels, and clams. It provides an average bioavailable fraction of a toxin over deployment time that can be used to determine an overall toxin risk to organisms. The sampling period typically depends on the bioactivity at a site, ranging from 24 hours to 4 weeks in most cases.
A SPATT passively absorbs and desorbs extracellular compounds over its stretch of time at a sampling site; in an organism, a toxin would go through biochemical detoxification processes. Passive samplers have a higher sensitivity for more compounds and provide improved stability and preservation of these compounds within the resin. SPATT devices capture less commonly detected cyanotoxins (e.g. cylindrospermopsin) at lower concentrations than that of a grab sample (collected at one point in time). Grab samples are limited in scope and sensitivity, and underrepresent toxins like microcystin-LR, which is picked up very reliably through SPATT technology.
Uses HP20 that is widely applicable for many toxins.
Used to capture:
Set of three Solid Phase Adsorption Toxin Tracking (SPATT) Bags
Pre activated
Ready for deployment
HP20
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map