Introduction

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Details

Specifications

Features	Specifications
Main Functions	Extract viral RNA/DNA from non-cell/low cell content biological samples
Applications	RT-PCR，PCR，NGS
Products	Viral total nucleic acid
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Cell-free body fluid such as plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount	200μl
Yield	2-10μg
Elution volume	≥30μl
Time per run	≤30 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA / RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).

Advantages

• Fast – several samples can be extracted in 20 minutes by column method
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG
• High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

Contents	IVD4173
Purification Times	100 Preps
HiPure Viral Mini Column	100
2ml Collection Tubes	200
PK/Carrier RNA	50 mg
Protease Dissolve Buffer	5 ml
Buffer AL	30 ml
Buffer MW1*	44 ml
Buffer MW2*	50 ml
RNase Free Water	15 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

Experiment Data

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

GL20 Technical Parameter：

Max. Speed	21000rpm
Max. RCF	30910×g
Max. Capacity	4×800ml
Time Range	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 60
Temperature	-20℃~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	680×540×830mm
Net Weight(Kg)	170KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for GL20

Order No	Rotor	Rotor Max Speed(r/min)	Volume(ml)	RCF(×g)
G20-1	Swing Rotor	4000	4×800ml	3450
G20-2	Microplate rotor	4000	4×4×96 well	2940
G20-3	Microplate rotor	4000	2×4×96 well	3210
G20-4	Microplate rotor	4000	2×3×48 well	2300
G20-5	Swing rotor	4000	4×40×7ml vacuum tube	3270
G20-6		4000	4×40×5ml vacuum tube	2890
G20-7		4000	4×28×10ml vacuum tube	3130
G20-8	Swing Rotor	4000	Fat bottle	3830
G20-9	Fixed Rotor	16000	4×8PCR	15760
G20-10	Fixed Rotor	15000	6×8PCR	21420
G20-11	Fixed Rotor	16000	8×8PCR	17480
G20-12	Fixed Rotor	15000	12×8PCR	22930
G20-13	Fixed Rotor	15000	40×0.5ml	22920
G20-14	Fixed Rotor	21000	12×1.5ml	30910
G20-15	Fixed Rotor	16000	24×1.5ml	23440
G20-16	Fixed Rotor	14000	30×1.5ml	20800
G20-17	Fixed Rotor	13000	48×1.5ml	17930
G20-18	Fixed Rotor	16000	16×5ml	22020
G20-19	Fixed Rotor	16000	6×10ml	21500
G20-20	Fixed Rotor	15000	12×10ml	22680
G20-21	Fixed Rotor	13000	16×10ml	19490
G20-22	Fixed Rotor	13000	8×15ml	17790
G20-23	Fixed Rotor	11000	12×15ml	14330
G20-24	Fixed Rotor	5000	24×15ml	3500
G20-25	Fixed Rotor	5000	30×15ml	3830
G20-26	Fixed Rotor	14000	6×30ml	19060
G20-27	Fixed Rotor	13000	6×50ml(conical)	18840
G20-28	Fixed Rotor	13000	6×50ml(round)	18730
G20-29	Fixed Rotor	5000	12×50ml	3860
G20-30	Fixed Rotor	4000	24×50ml	2970
G20-31	Fixed Rotor	13000	4×85ml	18940
G20-32	Fixed Rotor	12000	4×100ml	14850
G20-33	Fixed Rotor	10000	6×100ml	11380
G20-34	Fixed Rotor	4000	12×100ml	2970
G20-35	Fixed Rotor	12000	24 capillaries	15800
G20-36	Swing Rotor	15000	4×5ml	19920
G20-37	Vertical rotor	16000	16×5ml	16450
G20-38	Vertical rotor	14000	8×30ml	19750

Features

1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.

2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control

3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.

4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.

6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect inner sleeve , 3 tiers protection,safe and reliable.

7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.

Description

For rapid, sensitive and accurate screening of potential Tyrosinase inhibitors

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Tyrosinase (EC 1.14.18.1) is a copper-binding enzyme that is expressed across a vast range of species ranging from bacteria and fungi to mammals. It is involved in two sequential reactions of the melanin synthesis pathway: first being the hydroxylation of a monophenol and second the conversion of an ortho-diphenol to a quinone. Quinone then undergoes a series of reactions including polymerization to form melanin.

Tyrosinase is of great interest to the agriculture industry since it causes browning of fruits, vegetable, and mushrooms, as well as to the cosmetic industry as it causes skin darkening. Development and screening of tyrosinase inhibitors, therefore, is very useful for conditions such as hyperpigmentation and melasma. Tyrosinase activity is significantly increased in melanoma. Therefore, the detection of tyrosinase activity could be promising as a specific diagnostic test for melanoma and may be useful in monitoring patient response to melanoma treatments.

This Tyrosinase Activity Assay Kit is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at 520 nm. The assay can detect as low as 30 μU Tyrosinase in biological samples.

Highly reproducibility
Highly Sensitive Assay to Screen for Tyrosinase Activity
Stable formulation of ready to use Reaction Facilitator (tyrosinase)