Sulfo DBCO-Amine can be used to derivatize carboxyl-containing molecules or activated esters (e.g. The NHS ester) with DBCO moiety through a stable amide bond. The low mass weight will add minimal spacer to modified molecules and the hydrophilic sulfonated spacer arm will greatly improve water solubility of DBCO derivatized molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-Amine can be used to derivatize carboxyl-containing molecules or activated esters (e.g. The NHS ester) with DBCO moiety through a stable amide bond. The low mass weight will add minimal spacer to modified molecules and the hydrophilic sulfonated spacer arm will greatly improve water solubility of DBCO derivatized molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Product Description
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | DNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
DD5 Low Speed 5000rpm Swing rotor 4x800ml lab Centrifuge Adapters for 5/10/15/50ml tubes.
DD5 Low Speed Large Capacity Centrifuge Application:
DD5 Lab centrifuge widely used in the fields of pharmaceutical factory and laboratory, can hold swing out rotors and angle rotors both.
DD5 Low Speed Large Capacity Centrifuge Features:
1. Brushless frequency motor which in great torque, free maintenance, no powder pollution, quick in speed up and down.
2. Digital display which indicates the program, speed, time, RCF and temperature.
3. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration/deceleration for your choice.
4. There are kinds of rotors for your choice.
5. Electric lid lock, compact design, super speed and imbalance protection.
6. The centrifuge body is made of high-quality steel, safe and reliable.
DD5 Low Speed Laboratory Centrifuge Technical Parameters:
| Max. Speed | 5000rpm |
| Max.RCF | 4730xg |
| Max.Capacity | 4x800ml |
| Timer | 0~99mins |
| RPM/RCF Convert | Yes |
| Noise(DB) | ≤58 |
| Acc/Dec | 10 Rates |
| Speed Accuracy | ±20rpm |
| Voltage(V/HZ) | AC 220V/110V 50HZ/60HZ |
| Dimension(LxWxH mm) | 720x540x830 |
| Net Weight(Kg) | 108kg |
| Certificates | CE,ISO |
| Warranty | 1 Year |
DD5 Low Speed Laboratory Centrifuge Matched Rotors:
| Order No. | Rotor type | Max.Speed (rpm) | Max.Volume (ml) | Max.RCF (xg) |
| No30671 | Swing out rotor | 4,000 | 4x800ml(round cup) | 3,450 |
| No30679 | Swing out rotor | 4,000 | 4x500ml(square cup) | 3,450 |
| No30696 | Swing out rotor | 4,000 | 4x500ml(round cup) | 3,530 |
| No31377 | Swing out rotor | 5,000 | 4x50ml | 4,730 |
| 4x100ml | 4,730 | |||
| No30592 | Swing out rotor | 4,000 | 4x30x7ml | 3,140 |
| 4x40x7ml | 3,270 | |||
| 4x18x10ml | 3,140 | |||
| No31491 | Mircoplater rotor | 4,000 | 2x4x96well | 2,910 |
| No31494 | Microplater rotor | 4,000 | 4x4x96well | 2,940 |
| No30627 | Angle rotor | 5,000 | 30x15ml | 3,830 |
| No30641 | Angle rotor | 5,000 | 12x50ml | 3,860 |
| No30642 | Angle rotor | 4,000 | 24x50ml | 2,970 |
| No30614 | Angle rotor | 5,000 | 6x100ml | 3,130 |
| No30643 | Angle rotor | 4,000 | 12x100ml | 2,970 |
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PEG3-(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane)-(Amino-Tri-(carboxyethoxymethyl)-methane) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid groups can react with primary amino groups in the presence of activators (e.g. HATU) to form a stable amide bond.
PEG3-(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane)-(Amino-Tri-(carboxyethoxymethyl)-methane) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid groups can react with primary amino groups in the presence of activators (e.g. HATU) to form a stable amide bond.
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