Sulfo DBCO-Amine can be used to derivatize carboxyl-containing molecules or activated esters (e.g. The NHS ester) with DBCO moiety through a stable amide bond. The low mass weight will add minimal spacer to modified molecules and the hydrophilic sulfonated spacer arm will greatly improve water solubility of DBCO derivatized molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-Amine can be used to derivatize carboxyl-containing molecules or activated esters (e.g. The NHS ester) with DBCO moiety through a stable amide bond. The low mass weight will add minimal spacer to modified molecules and the hydrophilic sulfonated spacer arm will greatly improve water solubility of DBCO derivatized molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.
Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.
Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.
The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.
Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.
Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.
TruScript Reverse Transcriptase is Available in Three Convenient Formats:
1. TruScript Reverse Transcriptase Kit (Cat.#54440)
This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.
2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.
3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.
| TruScript Reverse Transcriptase | TruScript First Strand cDNA Synthesis Kit for mRNA | TruScript First Strand cDNA Synthesis Kit | |
| Kit contains only reverse transcriptase enzyme and buffer (no primers or other buffer components) | |||
| Your template is enriched mRNA | |||
| Your template is total RNA (poly A OR non-poly A-containing transcripts) | |||
| Kit contains oligo dT | |||
| Kit contains both oligo-dT primer and Random Hexamers | |||
| Kit contains reaction buffer with nucleotides and primer | with oligo-dT | with oligo-dT and random hexamers | |
| You have your own first strand synthesis primer | |||
| Your template is microRNA (using your own primers) | |||
| Cat.#54440 | Cat.#54400 | Cat.#54420 |
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TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.# 54400) – Kit Components
1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert
Storage Conditions and Product Stability
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 54440 (10,000 units) | Cat. 54420 (50 rxns) | Cat. 54410 (50 rxns) |
|---|---|---|---|
| TruScript™ Reverse Transcriptase (200 units/ μL) | 50 μL | – | – |
| 5x RT Buffer | 1 mL | – | – |
| TruScript™ Enzyme Mix | – | 100 μL | 100 μL |
| 2x Reaction Mix | – | 500 μL | – |
| 2x Reaction Mix for mRNA | – | – | 500 μL |
| Nuclease-Free Water | 1.25 mL | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 | 1 |
