DBCO-PEG5-DBCO is a bifunctional click chemistry reagent. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG5-DBCO is a bifunctional click chemistry reagent. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
dsDNA Quantification BR Kit
The kit includes BR Dye, BR Dilution Buffer, and two DNA Standards. The assay is accurate for DNA concentrations from 100 pg/µL to 1000 ng/µL, and is highly selective for double-stranded DNA over RNA.
Features
DNA selectivity and sensitivity. A series of input DNA (200, 400, 600, 800, and 1000 ng) was used with or without RNA contamination.
Cat.# 40041 dsDNA Quantification BR Kit, 100 assays and 500 assays
DBCO-PEG4-biotin is a click chemistry biotinylation that can enable copper-free click chemistry with azide molecules to form a stable triazole linkage. PEG4 spacer increases aqueou solubility of the molecules conjugated to the biotin compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-biotin is a click chemistry biotinylation that can enable copper-free click chemistry with azide molecules to form a stable triazole linkage. PEG4 spacer increases aqueou solubility of the molecules conjugated to the biotin compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) offers a PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations. The kit consists of a specially designed primer mix compatible with the Illumina system, that is used in conjunction with the provided 2X Real-Time PCR Master Mix to amplify a library of unknown concentration. The unknown library is accurately quantified by using a standard curve constructed from the provided DNA Standard (range from 20 pM to 2 fM) on a Real-Time PCR System. The kit is specially optimized to quantify Small RNA-Seq libraries with DNA standards that have similar size to a Small RNA-Seq library. However, it could also be used with other types of NGS libraries.
Figure 1 / 2
Click for expanded view
Storage Conditions
Upon receipt, store Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
| Component | Cat. 61600 (100 reactions) |
|---|---|
| 2X Real-Time PCR Master Mix | 1 mL |
| NGS Library Quantification Primer Set Mix | 600 µL |
| Quantified NGS Library Standards (for Small-RNA Seq)* | 5 x 80 µL Standards |
| Product Insert | 1 |
* Quantified Library Standards are provided as 20 pM, 2 pM, 200 fM, 20 fM and 2 fM
