Bis-PEG11-DBCO represents a homobifunctional PEG linker with DBCO moieties on either side that perform copper-free catalyzed click chemistry with azides. The PEG spacer imparts water-solubility and helps with fine-tuning DMPK properties. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-PEG11-DBCO represents a homobifunctional PEG linker with DBCO moieties on either side that perform copper-free catalyzed click chemistry with azides. The PEG spacer imparts water-solubility and helps with fine-tuning DMPK properties. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Thermal adapter for deep well plates, e.g., NEST 96 Deepwell Plate 2 mL. Compatible with the Opentrons Heater-Shaker Module.
Thermal adapter for deep well plates, e.g., NEST 96 Deepwell Plate 2 mL. Compatible with the Opentrons Heater-Shaker Module.
MagPure Plant DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and tissues.The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo.Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridization, and transgenosis detection.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 50-100 mg plant, fungal tissue |
| Applications | PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Conventional economic plant samples |
| Sample amount | Fresh / frozen plant samples:≤ 100mgDried plant / seed samples:≤ 20mg |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
| Contents | D635101 | D635102 | D635103 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure Particles | 1.7 ml | 4 ml | 18 ml |
| RNase Solution | 0.6 ml | 1.2 ml | 6 ml |
| Buffer SPL | 30 ml | 60 ml | 300 ml |
| Buffer PS | 10 ml | 20 ml | 100 ml |
| Buffer GW1* | 26 ml | 53 ml | 220 ml |
| Elution Buffer | 10 ml | 30 ml | 120 ml |
Storage and Stability
RNase Solution and MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
MagPure Plant DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and tissues.The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo.Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridization, and transgenosis detection.
Product Description
Kit Storage and Term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal Nucleic Acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex;perform a homology search and bind the target homology domain,at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation,the polymerase also binds to the 3′ end of the primer, initiating chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.
Isothermal Nucleic Acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | RNA Isothermal Amplification Kit EXO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal Nucleic Acid Applications
Suitable for RNA isothermal rapid amplification kit(Fluorescent type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
