DBCO-PEG12-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG12-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-GLULQR
SKU: 700007404
50 assays (manual) / 500 assays (auto-analyzer)
| Content: | 50 assays (manual) / 500 assays (auto-analyzer) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Analyte: | D-Glucose |
| Assay Format: | Spectrophotometer, Auto-analyzer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 1 to 150 µg of D-glucose per assay |
| Limit of Detection: | 2 mg/L |
| Limit of Quantification: | 6 mg/L |
| Reproducibility (%): | < 5% |
| Reaction Time (min): | ~ 5 min |
The D-Glucose (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of D-Glucose in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.
Browse more of our monosaccharide and oligosaccharide assay kits.
Advantages
The D-Glucose (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of D-Glucose in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.
HiPure PCR Pure Micro Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments between 60bp-20kbp from PCR products and enzymatic reaction solution |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | PCR products, enzymatic reaction solution |
| Sample amount | 10-200μl |
| Recovery | ≥80% |
| Elution volume | ≥7μl |
| Time per run | ≤10 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 10µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D212002 | D212003 |
| Purification Times | 100 Preps | 250 Preps |
| Buffer DP | 60 ml | 120 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Elution Buffer | 15 ml | 30 ml |
| HiPure DNA Micro Columns | 100 | 250 |
| 2 ml Collection Tubes | 100 | 250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
HiPure PCR Pure Micro Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Campylobacter jejuni is a curved, rod-shaped and microaerophillic gram negative bacterium. It is one of the most common causal agents of gastroenteritis with diarrhea as the main symptom. While infection of C. jejuni is seldom life-threatening, it is considered one of the most common food-borne bacteria with over 2 million people infected per year in US alone. Infection of C. jejuni usually results from consumption of poorly prepared food including undercooked meat (particularly poultry), untreated water or raw unpasteurized milk. Traditional identification of C. jejuni involves culturing, however the microaerophilic characteristic of this bacterium make the enrichment process laborious and costly.
Campylobacter jejuni TaqMan PCR Kit, 100 reactions
Campylobacter jejuni TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM36150 (100 preps) | Cat. TM36110 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Campylobacter jejuni Primer & Probe Mix | 280 μL | 280 μL |
| Campylobacter jejuni Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
