Introduction

Usages:
For enumerating and enriching nonfastidious or fastidious bacteria.

Principle:
Tryptone, peptone and yeast extract multivalent powder provides a nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance.

Formulation（per liter）:
Pancreatic Digest of Casein            15g
Papaic Digest of Soybean              5g
Sodium chloride                     5g
Agar                              15g
Final pH 7.3±0.2  

How to use：
1.Suspend 40g in 1L of distilled water , stirring heated to boiling ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle

250g

K-ISOC

SKU: 700004306

100 assays (manual) / 1000 assays (microplate) / 1000 assays (auto-analyser)

Content:	100 assays (manual) / 1000 assays (microplate) / 1000 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	D-Isocitric Acid
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	1 to 80 µg of D-isocitric acid per assay
Limit of Detection:	0.35 mg/L
Reaction Time (min):	~ 3 min
Application examples:	Fruit juices, fruit products, soft drinks and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by EN, NEN, NF, DIN, GOST, IFU and AIJN

The D-Isocitric Acid test kit is for the specific and rapid measurement and analysis of D-isocitric acid in fruit juices.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

View our full list of organic acid assay kit products.

Advantages

• Very competitive price (cost per test) 
• All reagents stable for > 2 years after preparation 
• No wasted isocitrate dehydrogenase solution (stable suspension supplied) 
• Very rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Suitable for manual, microplate and auto-analyser formats

The D-Isocitric Acid test kit is for the specific and rapid measurement and analysis of D-isocitric acid in fruit juices.

Introduction

This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 1-100ul blood, FFPE, tissue and other samples
Applications	Second generation sequencing, PCR, real timePCR, etc.
Purification method	Monodisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount	Body fluid : 10 – 200μl, Tissue : ≤10mg
Yield	10ng – 15μg
Elution volume	80 -100μl
Time per run

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
• High purity – carboxyl magnetic beads with weak surface adsorption, getting higher purity
• General – can be used for various clinical samples

Kit Contents

Contents	IVD3101
Purification Times	200
MagBind Particles	4.5 ml
Proteinase K	90 mg
Protease Dissolve Buffer	10 ml
Buffer ATL	60 ml
Buffer AL	60 ml
Buffer BD*	20 ml
Buffer BXW1*	110 ml
Elution Buffer	30 ml

Storage and Stability

Proteinase K, MagBind Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 18 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.