TCO-PEG2-DBCO is a short PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO is a click chemistry handle which easily reacts with azides, while the TCO function readily reacts with tetrazine-containing compounds.
TCO-PEG2-DBCO is a short PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO is a click chemistry handle which easily reacts with azides, while the TCO function readily reacts with tetrazine-containing compounds.
TCO-PEG2-DBCO is a short PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO is a click chemistry handle which easily reacts with azides, while the TCO function readily reacts with tetrazine-containing compounds.
Sample type purification kit guide
The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Figure 1 / 3
Click for expanded view
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70200 (24 preps) | Cat. 70210, 70220, 70230, 70240 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V1-V3 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
K-SORB
SKU: 700004339
58 assays (manual) / 580 assays (microplate) / 700 assays (auto-analyser)
| Content: | 58 assays (manual) / 580 assays (microplate) / 700 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Sorbitol, D-Xylitol |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 492 |
| Signal Response: | Increase |
| Linear Range: | 1.0 to 20 µg of D-sorbitol (or xylitol) per assay |
| Limit of Detection: | 0.20 mg/L |
| Reaction Time (min): | ~ 15 min |
| Application examples: | Diabetic foods (e.g. honey, jam and chocolate), dietetic foods, chewing gum, candies, fruit juice (e.g. apple juice), ice-cream, sweets, bakery products (e.g. desserts), marzipan, paper (and cardboard), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by IFU and AIJN |
The D-Sorbitol/Xylitol test kit for the determination Of D-Sorbitol And Xylitol In Foodstuffs, such as bakery goods, diabetic foods, chocolate, fruit, sweets and more.
Sorbitol is non-cariogenic and finds many dietetic applications, but can cause gastrointestinal problems in adults if large quantities are consumed (10-50 g per day). It has been safely used in processed foods for almost half a century and finds applications in the cosmetics and pharmaceuticals industries.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See our full range of alcohol test kits.
Advantages
The D-Sorbitol/Xylitol test kit for the determination Of D-Sorbitol And Xylitol In Foodstuffs, such as bakery goods, diabetic foods, chocolate, fruit, sweets and more.
Norovirus is a single-stranded, non-enveloped RNA virus belonging to the Caliciviridae family. Norovirus is considered the major causative agent of non-bacterial gastroenteritis in the world. The main channel of transmittance is via contaminated food or water, as well as human-to-human contact. Most Noroviruses infecting humans belong to genogroup I and II (particularly genotype 4). Norovirus is very stable in the environment and is resistant to some surface disinfectants such as alcohols and detergents. Moreover, it is very difficult to culture Norovirus in vitro, making its identification by standard microbiological assays challenging.
NoV TaqMan RT-PCR Kit, 100 reactions
NoV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM41450 (100 preps) | Cat. TM41410 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| NoV Primer & Probe Mix | 280 μL | 280 μL |
| NoV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |