TCO-PEG8-DBCO

Overview

• Detection kits for Streptococcus agalactiae
• Lyophilized format designed for room temperature shipping
• Available in TaqMan format for analysis

Norgen’s Streptococcus agalactiae TaqMan PCR Lyophilized Kit is designed for the detection of Streptococcus agalactiae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s Streptococcus agalactiae TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Streptococcus agalactiae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

Supporting Data

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Storage Conditions and Product Stability

• All kit components should be stored at -20°C upon arrival.
• Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
• Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
• All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

Component	Cat. TM30650L (100 preps)	Cat. TM30610L (100 preps)
MDx TaqMan 2X PCR Master Mix (Lyo)	4 x 350 μL	–
Streptococcus agalactiae Primer & Probe Mix (Lyo)	280 μL	280 μL
Streptococcus agalactiae Positive Control (Lyo)	150 μL	150 μL
Nuclease-Free Water (Negative Control)	3 x 1.25 mL	1 x 1.25 mL
Product Insert	1	1

Overview

Pierceable heat sealing foil which has a high solvent resistance. This seal is resealable, suitable for PCR, compound storage and sample shipping.

• Heat sealing offers a 100% effective method of plate sealing, for complete seal integrity, as well as being quick and cost effective
• PierceASeal Foil is compatible with polypropylene and polystyrene plates
• This seal demonstrates good solvent resistance and can be used for low temperature and room temperature compound storage in DMSO and organic solvents
• PierceASeal Foil can be pierced with a pipette tip manually, or by a liquid handling robot
• This seal can be resealed by applying another PierceASeal Foil seal straight on top of a previously pierced seal
• This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
• A green dotted line on the sheet foils clearly indicates the non-sealing surface, for ease of seal orientation and handling

Pierceable heat sealing foil which has a high solvent resistance. This seal is resealable, suitable for PCR, compound storage and sample shipping.

Overview

• Quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range
• Compatible with any Real-Time PCR system
• RNA is accurately quantified using a standard curve constructed from the provided RNA standard

Norgen’s Low Abundance RNA Quantification Kit offers a PCR-based detection procedure to quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range. The kit has two main enzymatic components – reverse transcription using Norgen’s microScript Reverse Transcription system and PCR Master Mix used in conjunction with  a specially formulized primer mixture, to amplify human RNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown RNA is accurately quantified by using a standard curve constructed from the provided RNA Standard. 

Details

Supporting Data

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Storage Conditions
Upon receipt, store Norgen’s Low Abundance RNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.

Component	Cat. 58900 (48 reactions)
microScript microRNA Enzyme Mix	20 μL
2x microScript Reverse Transcriptase Reaction Mix	0.5 mL
2X Real-Time PCR Master Mix	1 mL
RNA Quantification Primer Set Mix	200 μL
Quantified RNA Standard 50 pg/μL	2 x 60 μL
Nuclease-Free Water	3 x 1.25 mL
Product Insert	1