Methyltetrazine-PEG1-DBCO

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected*. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings

Overview

• Detection kits for Staphylococcus aureus
• Available in TaqMan format for analysis

Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by bacteria, and Staphylococcus aureus is often considered the most common cause of contagious mastitis in dairy herds. S. aureus infection is estimated to be present in up to 90% of dairy farms and is responsible for 35% of the economic loss in the dairy industry (Lee et al., 2005). S. aureus is a facultatively anaerobic, Gram positive bacterium. The majority of S. aureus strains are catalase-positive and coagulasepositive, which forms the basis of traditional identification methodology.

Staphylococcus aureus TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Staphylococcus aureus TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM29350 (100 preps)	Cat. TM29310 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
S. aureus Primer & Probe Mix	280 μL	280 μL
S. aureus Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description

About the genesig q32 Real-Time PCR Instrument

genesig 32 Overview
The genesig q32 Real-Time PCR Instrument is one of the fastest qPCR instruments on the market today due to its rapid heating and cooling. Assembled from just a few building blocks, this robust qPCR instrument allows analysis of up to 32 samples in both tube or strip format. The powerful genesig q32 software is easily installed from a USB flash drive onto any PC or Mac. The genesig q32 is used with 550 genesig Real-Time PCR Kits for human, vet, and food pathogens, as well as food speciation testing

The genesig Real-Time PCR Kit product range includes tests for a massive range of applications:

• Human Infectious Disease Screening
• Veterinary Diagnostics
• Food and Water testing
• Biothreat Detection

genesig q32 – Cutting edge design and technology
Assembled from just a few building blocks the robust genesig q32 system is easy to transport and install. Up to 32 samples can be run in 0.1 ml tubes or 8-strip format. Fast heating and cooling is achieved by utilizing robust Peltier elements, whilst assay performance is supported further by a heated lid design. Excitation is provided by high intensity LEDs filtered to provide light at 500nm that is capable of exciting all fluorophores commonly used in qPCR. A prism is used to generate spectra from fluorescent emissions. These spectra are imaged using a CMOS camera.

genesig q32 software
The genesig q32 software is delivered and easily installed from a USB flash drive. When installed in a network, the software can control several q32 instruments connected via LAN. The powerful and easy to use software provides the following features:

• Quick start using templates for all genesig kit applications
• Straightforward setup and editing of sample and target information
• Automated analysis modules for absolute and relative quantification, melting curve analysis and endpoint genotyping
• Analysis of full spectral data
• Compatible with Windows, Mac & Linux Systems
• Instrument start from a USB flash drive, using preprogrammed settings
• Data export functions

genesig Real-Time PCR Kit compatible
genesig Real-Time PCR Kits are suitable for use on the genesig q32 instrument. The genesig Kit range includes over 550 kits for human, vet, and food pathogens, as well as food speciation testing. All genesig Kits include a positive control and resuspension buffers. The kits come freeze-dried so that they can be shipped at room temperature for fast delivery to anywhere in the World.

Automated run templates available for:

• genesig Easy Real-Time PCR Kits
• genesig Advanced Real-Time PCR Kits
• genesig Standard Real-Time PCR Kits
• snpsig Real-Time PCR Kits
• quasa Real-Time PCR Kits
• GMO Real-Time PCR Kits

Dimensions: W 24 cm x D 27 cm x H 23 cm
Weight: 7 kg
Operating noise: Electrical: Voltage: 100 – 240 V ( 10%); Frequency: 50 – 60 Hz ( 10%); Power: 170 W
Number of reactions: 32
Reaction vessels: 0.1ml tube and/or 8-tube strips
Reaction volume: 10 – 100 μl (20 μl recommended)
Temperature cycling system: Peltier-based heating & cooling from 40 to 99°C; Heating rate: 4°C/s & Cooling rate: 3°C/s
Temperature uniformity:  /- 0.05°C (SD)
Temperature accuracy:  /- 0.25°C
Run time: Fluorescence excitation: 500 nm nm (blue LED)
Fluorescence detection: 120 optical channels from 510 to 750 nm (CMOS camera)
Factory-calibrated dyes (at shipment): FAM; VIC / HEX
Applications: Relative and absolute quantification analysis; Melting peak analysis; Endpoint genotyping analysis
Sensitivity: 1.1 fold discrimination; 9-log dynamic range; Single copy detection
Connection options: LAN; Direct connection to computer (RJ45); PC-free (USB stick/flash drive controlled)Candidatus Liberibacter asiaticus Manual

Guaranteed results using 550 genesig kits
Fast DNA and RNA analysis in less than 60 minutes
Quick and easy set-up and run
Automated data analysis result calling
Small and robust instrument
Exceptional value for money