Methyltetrazine-PEG4-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Methyltetrazine-PEG4-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
| Clone | IHC697 |
| Source | Mouse Monoclonal |
| Positive Control | Colon Cancer |
| Dilution Range | 1:200 |
Thymidylate Synthase (TS) is a crucial enzyme responsible for the synthesis of 2′-deoxythymidine-5′-monophosphate (dTMP) a precursor for thymidylate which is necessary for DNA replication and repair from 2′-deoxyuridine-5′-monophosphate (dUMP). In terms of cancer, TS is an important target for cancer treatment as the inhibition of TS and therefore nucleotide synthesis necessary for cell growth has shown to be a vital part for successful treatment against colorectal, pancreatic and breast cancers.
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Specifications
| Features | Specifications |
| Main FunctionsC | Co-isolation DNA and RNA from a single FFPE tissue sample |
| Applications | RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | FFPE slice, FFPE embedded tissue |
| Sample amount | No more than six 10µm sections of 150mm2 surface area or three 20µm sections of 150mm2 surface area. |
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. For RNA purification, transfer RNA Lysate to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer. For DNA purification, transfer DNA Lysate to an adsorption column and DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA was finally eluted with low-salt buffer.
1.Use non-toxic dewaxing solution without contact with xylene
2.Obtain both DNA and RNA simultaneously from the same sample. Elute separately without affecting each other (Have the same steps and effects as top brand 80234, perfect substitute.)
| Contents | IVD5116 |
| Purification Times | 50 Preps |
| HiPure DNA Micro Column | 50 |
| HiPure RNA Mini Column I | 50 |
| 2ml Collection Tubes | 150 |
| Proteinase K | 50 mg |
| Protease Dissolve Buffer | 5 ml |
| Buffer DPS | 60 ml |
| Buffer FRL | 15 ml |
| Buffer ATL | 15 ml |
| Buffer RLC | 15 ml |
| Buffer AL | 15 ml |
| Buffer VHB | 44 ml |
| Buffer RW2 | 25 ml |
| RNase Free Water | 10 ml |
| Buffer AE | 10 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
SD-25/SD-25C Vacuum Centrifugal Concentrator
Ambient temperature type/Refrigerated type
Technical Parameter
| Temperature range | Room Temperature~+80℃ | -10℃~+80℃ |
| Temperature Accuracy | 士1℃ | |
| Max.Speed | 2500rpm | |
| Max.Capacity | 6x100ml | |
| Timer | 0~99h59min | |
| Vacuum control range | 0.1~1000mbar | |
| Main unit withstands vacuum levels | 0.01mbar | |
| Noise | ≤55dBA | ≤65dBA |
| Voltage | 0.7kw | 1.3kw |
| Power supply | 220v,50Hz | |
| Dimension(L×W×Hmm) | 360*448*304mm | 440*653*420mm |
Adapter Rotor
| Rotor Type | Rotor Volume | Rotor Type | Rotor Volume |
| Angle Rotor | 42×1.5/2ml | Angle Rotor | 8×50ml |
| Angle Rotor | 108×1.5/2ml | Angle Rotor | 6×100ml |
| Angle Rotor | 48×5ml | Angle Rotor | 16*20ml Sample vial |
| Angle Rotor | 30×10ml | Angle Rotor | 16*5ml+8*4ml+8*3ml |
| Angle Rotor | 24×15ml | Microplate Rotor | 2×96well |
Key features：
1. High-definition LCD touch screen, simultaneously displaying operational parameters: speed, temperature, vacuum, and running time.
2. Delayed start: after reaching a fixed speed, Start the vacuum pump to effectively prevent sample mixing.
3. Fully automatic vacuum control system, vacuum setting range0.1~1000mbar, Control accuracy0.1mbar.
4. 1.5/2ml Rotor stacking for simultaneous use; rotor replacement without tools.
5. Programmable with temperature and pressure settings; capable of storing 36 programs
6. Compatible with various rotor heads; transparent glass window for easy observation.
7. Magnetic drive: maintenance-free, non-contact drive, fully sealed.
8. Safety in use: inductive electric self-suction door lock ensures the centrifugal concentrator can only start when the door cover is fully closed and locked.
9. Can be used in conjunction with freeze-drying systems
Model:
SD-25/SD-25C
Brand:
Yingtai
