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Methyltetrazine-PEG3-DBCO

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Methyltetrazine-PEG4-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Detail

Methyltetrazine-PEG4-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Other Products

TruScript Reverse Transcriptase and Kits

Overview

  • Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction
  • Time Savings – Set up RT reactions in a shorter time since less pipetting steps are required
  • Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits
  • High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products
  • Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility

TruScript Reverse Transcriptase is Available in Three Convenient Formats:

1. TruScript Reverse Transcriptase Kit (Cat.#54440)

This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.

2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)

This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).

The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.

3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)

This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).

The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.

Which TruScript Kit is Best for Your Needs?

 TruScript
Reverse Transcriptase
TruScript
First Strand cDNA
Synthesis Kit
for mRNA
TruScript
First Strand cDNA
Synthesis Kit
Kit contains only reverse
transcriptase enzyme and
buffer (no primers or other
buffer components)
  
Your template is enriched
mRNA
  
Your template is total RNA
(poly A OR non-poly
A-containing transcripts)
 
Kit contains oligo dT  
Kit contains both oligo-dT
primer and Random
Hexamers
  
Kit contains reaction buffer
with nucleotides and primer
 
with oligo-dT
 

with oligo-dT and
random hexamers
You have your own first
strand synthesis primer
  
Your template is microRNA
(using your own primers)
  
 Cat.#54440Cat.#54400Cat.#54420

Details

Supporting Data

Figure 1 / 2

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Figure 1. Robust Reverse Transcription of All RNA Transcripts
Figure 1. Robust Reverse Transcription of All RNA Transcripts
Figure 2. TruScript Reverse Transcriptase and Kits

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TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.# 54400) – Kit Components

1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water 
4. Product Insert

Storage Conditions and Product Stability

Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C.  These reagents should remain stable for at least 1 year in their unopened containers.

ComponentCat. 54440 (10,000 units)Cat. 54420 (50 rxns)Cat. 54410 (50 rxns)
TruScript™ Reverse Transcriptase (200 units/ μL)50 μL
5x RT Buffer1 mL
TruScript™ Enzyme Mix100 μL100 μL
2x Reaction Mix500 μL
2x Reaction Mix for mRNA500 μL
Nuclease-Free Water1.25 mL1.25 mL1.25 mL
Product Insert111

Propargyl-PEG6-NHS ester

Propargyl-PEG6-NHS ester is an amine reactive linker. The alkyne in this linker can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reaction. The PEG spacer increases solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Human Influenza type B

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.