DOTA-PEG5-C6-DBCO is a PEG linker containing DOTA and DBCO moieties. DBCO is used in Click Chemistry reactions due to its high strain energy. The DOTA group can be ionized and is susceptible for chelating di- and trivalent cations. DOTA can also be used for imaging diagnostic techniques. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DOTA-PEG5-C6-DBCO is a PEG linker containing DOTA and DBCO moieties. DBCO is used in Click Chemistry reactions due to its high strain energy. The DOTA group can be ionized and is susceptible for chelating di- and trivalent cations. DOTA can also be used for imaging diagnostic techniques. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from tissue using economic salt out method |
| Applications | PCR, enzyme digestion, Southern hybridization,etc. |
| Purification technology | Salting out method |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal tissue |
| Sample amount | Unlimited |
| Elution volume | ≥100μl |
| Time per run | Variation with sample amount |
Principles
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.
| Contents | D331201 | D331202 | D331203 |
| Purification Times | 1 g | 5 g | 50 g |
| Proteinase K | 330 µl | 1.8 ml | 18 ml |
| Buffer WTL | 33 ml | 160 ml | 2 x 800 ml |
| Buffer PPS | 12 ml | 55 ml | 500 ml |
| RNase Solution | 330 µl | 1.8 ml | 18 ml |
| Buffer TE | 12 ml | 60 ml | 200 ml |
RNase Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Sample type purification kit guide
The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
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| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70100 (24 preps) | Cat. 70110, 70120, 70130, 70140 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V1-V2 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, tissue and culture cells.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 10ml blood and 1g tissue using Maxi column |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification method | Maxi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples |
| Sample amount | 3-10ml |
| Elution volume | ≥700μl |
| Time per run | ≤90 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 5mg |
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
| Contents | D311502 | D311503 |
| Purification Times | 10 | 50 |
| HiPure gDNA Maxi Columns | 10 | 50 |
| 50ml Collection Tubes | 20 | 100 |
| Buffer ATL | 120 ml | 550 ml |
| Buffer AL | 120 ml | 2 x 300 ml |
| Buffer GW1* | 53 ml | 220 ml |
| Buffer GW2* | 25 ml | 110 ml |
| RNase A | 40 mg | 180 ml |
| Proteinase K | 120 mg | 540 mg |
| Protease Dissolve Buffer | 12 ml | 50 ml |
| Buffer AE | 30 ml | 120 ml |
Storage and Stability
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
| Name | CAT NO | Sample amount | Leukocyte protocol* | Colum type | Elutio volume | Average yield | Time per run |
| HiPure Blood DNA Mini Kit | D3111 | 10-200μl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| HiPure Tissue&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure Tissue&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure Tissue&Blood DNA 96 Kit | D3117 | 1-200μl | 1ml | 96 well plate | 3-8μg/200μl |
*Note：Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction
Select the right purification kit to get impactful results：
Ⅰ. Purchase guide of Magen kits based on sample
Ⅱ. Cross purchase guide for Magen kits vs Other brands
For any customized product or OEM service, please contact us.
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, tissue and culture cells.
Details
