m-PEG4-DBCO is a click chemistry reagents which can enable so called copper-free Click Chemistry with azide to form a stable triazole. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
m-PEG4-DBCO is a click chemistry reagents which can enable so called copper-free Click Chemistry with azide to form a stable triazole. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
m-PEG4-DBCO is a click chemistry reagents which can enable so called copper-free Click Chemistry with azide to form a stable triazole. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method.
MUG permits the rapid detection of Escherichia coli when the medium is observed for fluorescence using a UV light. MUG is hydrolyzed by the enzyme β-glucuronidase which is produced by E. coli to yield a fluorescent end product 4- methylumbelliferone. Trypticase provides the essential nutrients. Lactose is the fermentable carbohydrate. Sodium chloride maintains the osmotic equilibrium. The medium has a strong buffering system to control the pH in the presence of fermentative action. The bile salts inhibit gram-positive bacteria especially the Bacillus species and faecal Streptococci.
| Ingredients | /liter |
| Trypticase or tryptose | 20 g |
| Bile salts No. 3 | 1.5 g |
| Lactose | 5 g |
| K2HPO4 | 4 g |
| KH2PO4 | 1.5 g |
| NaCl | 5 g |
| 4-methylumbelliferyl-β-D-glucuronide (MUG) | 50mg |
| pH6.9±0.2 at 25°C | |
Weigh 37g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until
completely dissolved, sterilize at 115℃ for 20min, cool to room temperature and set aside.
The following quality control strains were inoculated and cultured at 44.5℃±0.5℃ for 24h. The results are as follows:
| Quality control strains | Growth |
| Escherichia coli ATCC25922 | Blue-white fluorescence is produced under 366nm UV light |
| Salmonella typhimurium ATCC14028 | No fluorescence under 366nm UV light |
| Enterococcus faecalis ATCC29212 | Clear, no fluorescence |
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method. Principle and Interpretation MUG permits the rapid detec……
Sulfo DBCO-PEG3-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain and sulfo group increase water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-PEG3-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain and sulfo group increase water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
2,5-Dioxopyrrolidin-1-yl 2-(prop-2-yn-1-yloxy)acetate is a Click Chemistry reagent with a propargyl group and an NHS ester group. The propargyl group can react with biomolecules containing azide group via copper catalyzed Click Chemistry reaction. The NHS ester is an amine reactive group which can be used for derivatizing peptides, antibodies, amine coated surfaces, etc. Reagent grade, for research use only.
2,5-Dioxopyrrolidin-1-yl 2-(prop-2-yn-1-yloxy)acetate is a Click Chemistry reagent with a propargyl group and an NHS ester group. The propargyl group can react with biomolecules containing azide group via copper catalyzed Click Chemistry reaction. The NHS ester is an amine reactive group which can be used for derivatizing peptides, antibodies, amine coated surfaces, etc. Reagent grade, for research use only.