N-(Propargyl-PEG4-carbonyl)-N-bis(PEG1-acid)

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Sixteen discrete fragments ranging from 100 bp to 5000 bp
• Higher intensity reference bands at 500 bp and 1000 bp

The Norgen FullRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our FullRanger contains sixteen discrete fragments ranging from 100 bp to 5000 bp with higher intensity reference bands at 500 bp and 1000 bp. This Ladder is ideal for accurate visual determination in both large and small cloning applications.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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FullRanger 100 bp DNA Ladder (Cat# 11800) – 100 loads

Ladder Properties:
• Sixteen discrete bands, ranging from 100 bp to 5000 bp
• Higher intensity reference bands at 500 bp and 1000 bp

Fragment	Size (bp)	Mass (ng)
1	5000	55
2	4000	44
3	3000	33
4	2500	28
5	2000	22
6	1500	17
7	1000	50
8	900	35
9	800	31
10	700	43
11	600	24
12	500	56
13	400	16
14	300	22
15	200	15
16	100	11

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings 150 reactions

Description

African Swine Fever Virus (ASFV) is a widespread disease which infects members of the pig family(Suidae). Anumberoftick species are believed to be the vector for the disease,as well as being transmitted by raw pork and pig excrement [1]. After firstly being identified in Kenya in 1921, ASFV became endemic in sub-Saharan Africa, with regular outbreaks being reported across Europe, Asia and South America throughout the century [2]. More recently the virus was introduced in Georgia and spread throughout the region, as well as mass outbreaks occurring in China in 2018 [3].
ASFVistheonlymemberoftheAsfaridaefamily.ItisalargeenvelopeddoublestrandedDNA virus of icosahedral morphology with an average diameter of 200nm and isolates contain genomes between 170-190Kbp encoding for up to 167 open reading frames [2]. The morphology of ASFV consist of several concentric domains. An inner core contains the nucleoid coated with a thick protein layered core shell, which is surrounded by an inner lipid envelope , all of which is encompassed by the capsid [2]. ASFV begins its replication cycle in the nucleus of infected cells before moving to the cytoplasm where the majority of the replication takes place [2]. Gene transcription is highly regulated, with distinct classes of mRNA identified to accumulate at early, intermediate and late transcripts of the virus [2]. The disease induces acute haemorrhagic disease within its hosts, causing high fevers and skin haemorrhages, with death often occurring within ten days of clinical symptoms appearing [4].

References: 1: The Centre for Food Security and Public Health (2015), African Swine Fever. 2: Galindo, I. and Alonso, C., 2017. African swine fever virus: a review. Viruses, 9(5), p.103. 3: Zhou, X., Li, N., Luo, Y., Liu, Y., Miao, F., Chen, T., Zhang, S., Cao, P., Li, X., Tian, K. and Qiu, H.J., 2018. Emergence of African swine fever in China, 2018. Transboundary and emerging diseases, 65(6), pp.1482-1484. 4: Gallardo, C., Ademun, A.R., Nieto, R., Nantima, N., Arias, M., Martín, E., Pelayo, V. and Bishop, R.P., 2011. Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007. African Journal of biotechnology, 10(17), pp.3488-3497.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings