• Format: 96-well microtiter plate (12 test strips of 8 wells)
• Standards: 0 | 0.1 | 0.25 | 0.5 |1.0 | 2.6 ppb
• Incubation Time: 75 Minutes

Description

Brevetoxin: Competitive ELISA for the quantitative analysis of Brevetoxin in samples

Format: 96-well microtiter plate (12 test strips of 8 wells)

Standards: 0 ppb | 0.1  ppb | 0.25 ppb | 0.5 ppb | 1 ppb | 2.5 ppb

Incubation Time: 75 Minutes

Brevetoxins (PbTx) are a class of cyclic polyether compounds produced by certain algae such as Karenia brevis. K. brevis can produce Brevetoxins in large quantities during an algae bloom which then pose a major health threat and are important to monitor and mitigate. Brevetoxins are the causative agent of Neurotoxic Shellfish Poisoning (NSP).

FDA and EPA safety levels in regulations and guidance – 0.8 mg/kg for clams, mussels, oysters, and whole and roe-on scallops, fresh, frozen, or canned. – National Shellfish Sanitation Program Guide for the Control of Molluscan Shellfish.

Format: 96-well microtiter plate (12 test strips of 8 wells)
Standards: 0 | 0.1 | 0.25 | 0.5 |1.0 | 2.6 ppb
Incubation Time: 75 Minutes

Introduction

This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from FFPE using high bind beads
Applications	RT-PCR, northern blot, poly A purification, nucleic acid protection and in vitro translation, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Large quantities of solids
Sample amount	Appropriate
Elution volume	≥50μl
Time per run	30 – 120 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

1.Use non-toxic dewaxing solution without contact with xylene

2.Efficient removal of formaldehyde modification on DNA and improvement of PCR sensitivity

3. One of the best FFPE DNA extraction kits on the market, the same effect of paraffin slice extraction as top brand, and the effect of puncture sample extraction is even better than top brands

Kit Contents

Contents	D632301B	D632302B
Purification Times	48 Preps	96 Preps
MagBind Particles	1.1 ml	2 x 1.1 ml
RNase A	10 mg	20 mg
Proteinase K	24 mg	48 mg
Protease Dissolve Buffer	3 ml	6 ml
Buffer DPS	60 ml	100 ml
Buffer ATL	15 ml	30 ml
Buffer AL	15 ml	30 ml
Buffer BD*	6 ml	15 ml
Buffer BXW1*	13 ml	44 ml
Elution Buffer	15 ml	30 ml

Storage and Stability

RNase A, Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.