N-t-Boc-Aminooxy-PEG4-N-(PEG2-Propargyl) is a click chemistry crosslinker. The propargyl group is reactive with azide-containing compounds or biomolecules through copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG linker improves solubility in aqueous media.
N-t-Boc-Aminooxy-PEG4-N-(PEG2-Propargyl) is a click chemistry crosslinker. The propargyl group is reactive with azide-containing compounds or biomolecules through copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG linker improves solubility in aqueous media.
Introduction
A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. This kit can be easily used in manual and automated 96 or 384-well formats.
Specifications
| Features | Specifications |
| Main Functions | Recover 100-400μl DNA/ RNA from PCR products / enzymatic reaction solution / or crude DNA / RNA |
| Applications | Automatic sequencing, enzyme digestion, PCR and labeling |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Agarose Gel, PCR products, enzymatic reaction solution |
| Sample amount | 100-400µl |
| Recovery | 80% |
| Elution volume | ≥30μl |
The MagPure method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments 100bp and larger toparamagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
Kit Contents
| Contents | D500301 | D500302 | D500303 |
| Purification Times | 50 Preps | 500 Preps | 5000 Preps |
| Buffer AL | 10 ml | 60 ml | 550 ml |
| Buffer BD* | 5 ml | 25 ml | 2 x 100 ml |
| MagPure RNA Particles | 1.2 ml | 12 ml | 120 ml |
| RNase Free Water | 5 ml | 30 ml | 250 ml |
Storage and Stability
MagPure RNA Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. This kit can be easily used in manual and automated 96 or 384-well formats.
TD4 Serofuge/Immunity centrifuge
Features:
1. Microprocessor control, less noisily, it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, laboratory and biochemistry.
2. Brushless motor, free maintenance, no powder pollution, quick in speed up and down.
3. The flexible axle driven system which drive the rotor directly, smooth in operation and small vibration.
4. Micro computer control system, digital display the RCF，time and speed.
5. Electric lid lock, compact design, super speed and imbalance protection.
TD4 Technical Data:
| Max Speed | 4500rpm | Max RCF | 2260×g |
| Max volume | 12×7ml | Power supply | AC 220V 50HZ 1A |
| Timer | 0～99min | Noise | ≤55dBA |
| Dimension(DxWxH) | 485×320×255mm | Net weight | 23 KG |
| Speed accuracy | ±20rpm | Package | Carton box |
Matched Rotors for TD4:
| Rotor Name | Max. Speed | Max. Volume | Max RCF(×g |
| HLA rotor for Lymphocyte washing | 4500RPM | 12*2/1.5/0.5 | 2260*g |
| SERO rotor for erythrocyte washing | 3000RPM | 12*7/5ml(10-13*65-100) | 1000*g |
DBCO-Acid is a non-activated building block and adds minimal spacer to modified molecules. In the presence of activators such as EDC or HATU, this reagent can be used to derivatize amine-containing molecules through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-Acid is a non-activated building block and adds minimal spacer to modified molecules. In the presence of activators such as EDC or HATU, this reagent can be used to derivatize amine-containing molecules through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Tel : 081-875-1869 , 02-328-7179
Email : [email protected]
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