Overview

• Detection kits for ZIKV
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

The Zika Virus (ZIKV) is an emerging mosquito-borne virus that was first identified in Uganda in 1947 in Rhesus monkeys through monitoring of sylvatic yellow fever. During large outbreaks in French Polynesia and Brazil, national health authorities reported potential neurological and auto-immune complications of ZIKV disease. Agencies investigating the Zika outbreaks are finding an increasing body of evidence about the link between ZIKV and microcephaly. Infection with ZIKV may be suspected based on symptoms and recent history (e.g. residence or travel to an area where ZIKV is known to be present). Zika virus diagnosis can only be confirmed by laboratory testing for the presence of ZIKV RNA in the blood or other body fluids, such as urine or saliva.

ZIKV TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

ZIKV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

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Storage Conditions

All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM62250 (100 preps)	Cat. TM62210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
ZIKV Primer & Probe Mix	280 μL	280 μL
ZIKV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Introduction

This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from FFPE using high bind beads
Applications	RT-PCR, northern blot, poly A purification, nucleic acid protection and in vitro translation, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Large quantities of solids
Sample amount	Appropriate
Elution volume	≥50μl
Time per run	30 – 120 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

1.Use non-toxic dewaxing solution without contact with xylene

2.Efficient removal of formaldehyde modification on DNA and improvement of PCR sensitivity

3. One of the best FFPE DNA extraction kits on the market, the same effect of paraffin slice extraction as top brand, and the effect of puncture sample extraction is even better than top brands

Kit Contents

Contents	D632301B	D632302B
Purification Times	48 Preps	96 Preps
MagBind Particles	1.1 ml	2 x 1.1 ml
RNase A	10 mg	20 mg
Proteinase K	24 mg	48 mg
Protease Dissolve Buffer	3 ml	6 ml
Buffer DPS	60 ml	100 ml
Buffer ATL	15 ml	30 ml
Buffer AL	15 ml	30 ml
Buffer BD*	6 ml	15 ml
Buffer BXW1*	13 ml	44 ml
Elution Buffer	15 ml	30 ml

Storage and Stability

RNase A, Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.

Name of Product

Leishmania infantum – IgG ELISA

Catalog Number

AF 9500

Short Info

This ELISA kit is intended for the quantitative detection of IgG antibodies against Leishmania infantum in human serum.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

pNPP, λ=405 nm

Test Principle

Specific antibodies in the sample bind to Leishmania infantum antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.

12 x 8 (96 tests)
This ELISA kit is intended for the quantitative detection of IgG antibodies against Leishmania infantum in human serum.