N-Boc-N-bis(PEG4-Propargyl)

Introduction

Usages:

For pre-enrichment of Salmonella spp.

Principle:

Peptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride maintains osmotic equilibrium; potassium dihydrogen phosphate and disodium hydrogen phosphate as buffer.

Formulation(per liter):
Peptone: 10g
Sodium chloride:5g
Disodium hydrogen phosphate:3.5g
Potassium dihydrogen phosphate: 1.5g
Final pH7.2 ± 0.2

How to use:
1. Suspend 20g of product, adding 1L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ℃ for 15min, set aside.
2.Diluted and treated samples.

Quality control：
          　　

Item	The name and number of strain	Growth	Colony Color
1	Salmonella typhi CMCC (B) 50071	good	Cloudy broth
2	Salmonella typhimurium CMCC (B) 50115	good	Cloudy broth
3	Paratyphoid Salmonella CMCC (B) 50093	good	Cloudy broth

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle; 225ml*10bag/box

Reference:
1.GB/T4789.4-2003 People’s Republic of China national standards of food hygiene Examination of Salmonella microorganisms
2.GB/T4789.28-2003 People’s Republic of China national standards of food hygiene microbiological examination Staining, media and reagents
3.SN0170-92 People’s Republic of China Import and Export Commodity Inspection industry standard Salmonella food for export (including Arizona bacteria) test method
4.GB/T4789.4-2008 People’s Republic of China national standards of food hygiene Examination of Salmonella microorganisms
5.GB 4789.4-2010 national standards for food safety standards of food hygiene inspection and microbiological testing of salmonella People’s Republic of China
6.GB 13091-91 People’s Republic of China National Standard Test Method for Salmonella in feed
7.GB 4789.40-2010 national standards for food safety standards of food hygiene inspection microorganism People’s Republic of China E.sakazakii test
8. ISO 6579-2002 Microbiology of food and animal feeding stuffs —– Horizontal method for the detection of Salmonella spp.
9. ISO22964-2006 Milk and milk products —– Detection of Enterobacter sakazakii
10. ISO6785-2001 Milk and milk products —– Detection of Salmonella spp

250g

Overview

• Extract high quality & quantity total RNA including miRNA
• No phenol step required; isolate all RNA in one fraction
• Bind & elute all RNA irrespective of size or GC content, without bias
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Isolate from a wide variety of specimens
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Available in a variety of formats to suit your needs
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A₂₆₀/A₂₈₀ suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)

This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Isolate RNA after Purifying EVs and Exosomes

Ultracentrifugation, Exoquick, Filtration

Cat. #	Name	Elution Volume	Plasma/Serum	Urine	Cell-Culture Media
55000	Plasma/Serum RNA Purification Mini Kit	10 – 25 µL	50 µL – 1 mL	250 µL – 1 mL	5 – 10 mL
35300	Total RNA Purification Micro Kit	20 – 50 µL	1 – 4 mL	2 – 10 mL	10 – 20 mL
17200	Total RNA Purification Kit	50 – 100 µL	4 – 10 mL	11 – 30 mL	20 – 35 mL

Details

Supporting Data

Figure 1 / 13

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Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material:
Animal Cells	1 x 106 cells
Animal Tissues	10 mg
Blood	100 μL
Plasma/Serum	150 μL
Bacteria	1 x 109 cells
Yeast	1 x 108 cells
Fungi	40 mg
Plant Tissues	40 mg
Time to Complete 96 Purifications	30 minutes
Average Yields*
HeLa Cells (1 x 106 cells)	15 μg
E. coli (1 x 109 cells)	50 μg

* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 17200 (50 preps)	Cat. 37500 (100 preps)	Cat. 17250 (250 preps)	Cat. 17270 (500 preps)	Cat. 24300 (192 preps)	Cat. 24370 (576 preps)	Cat. 24350 (192 preps)	Cat. 24380 (576 preps)
Buffer RL	40 mL	2 x 40 mL	175 mL	350 mL	2 x 40 mL	350 mL	2 x 40 mL	350 mL
Wash Solution A	38 mL	2 x 38 mL	148 mL	1 x 148 mL 1 x 74 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL
Elution Solution A	6 mL	2 x 6 mL	30 mL	60 mL	2 x 20 mL	60 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	100	250	500	–	–	–	–
96-Well Isolation Plate	–	–	–	–	2	6	–	–
96-Well Isolation Plate (Deep Well)	–	–	–	–	–	–	2	6
Adhesive Tape	–	–	–	–	4	12	4	12
Collection Tubes	50	100	250	500	–	–
96-Well Collection Plate	–	–	–	–	2	6	–	–
96-Well Collection Plate (Deep Well)	–	–	–	–	–	–	2	6
Elution Tubes (1.7 mL)	50	100	250	500	–	–
96-Well Elution Plate	–	–	–	–	2	6	–	–
96-Well Elution Plate (Deep Well)	–	–	–	–	–	–	2	6
Product Insert	1	1	1	1	1	1	1	1