N-(Boc-PEG4)-N-bis(PEG4-propargyl) is a trifunctional chemical containing two terminal alkynes and a Boc-protected primary amine. The terminal alkynes are used in copper click chemistry with azides to form stable triazole linkages with the target molecule while the carboxylic acids are reactive towards alcohols and primary amines to form esters and amides respectively.
Detail
N-(Boc-PEG4)-N-bis(PEG4-propargyl) is a trifunctional chemical containing two terminal alkynes and a Boc-protected primary amine. The terminal alkynes are used in copper click chemistry with azides to form stable triazole linkages with the target molecule while the carboxylic acids are reactive towards alcohols and primary amines to form esters and amides respectively.
Other Products
DNA Isothermal Amplification Kit NFO With 500-1000 Copies/UL Detection Limit 48 Tests / Box
Product Info
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Product Info
Product Description
Amp-future DNA Isothermal Amplification Kit NFO with 500-1000copies/µL Detection Limit, 48 Tests/box, -20°C Storage
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit NFO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
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Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
* Optical system: equipped with infinite flat field optical system, the whole machine mildew prevention design and large field eyepiece, with beautiful appearance, clear imaging, broad vision, convenient operation and other characteristics, can be widely used in biology, medicine, industry, agriculture and other fields, is the ideal instrument for medical, teaching, scientific research and other units
* Observation head device: the cylinder can rotate 360 degrees, the displacement of the center of the mirror is 0.06mm; 30 tilt; the hinge type binocular observation (the magnification difference between left and right systems is 2.0%; the end position difference is 0.1mm at zero vision) and the third party connection port, the pupil distance adjustment range is 50-75mm, the fixed observation cylinder head is internal hexagon screw or select portable screw.
* Eyepiece configuration: random eyepiece WF10X / 22mm; the magnification accuracy of eyepiece shall not exceed ± 2%. There is a card slot on the eyepiece tube to lock the eyepiece to prevent falling. Binocular vision is adjustable, making binocular observation easier;
* Loading platform: triangular rail XY composite mechanical moving loading platform; 0.005 lateral to 5N horizontal force, corrosion resistance and wear resistance. Rectangular, size 190X140mm, stroke 6090mm, minimum read value 0.1 mm.
Parameter of microscope
Specification configuration
TL36A
Binocular observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm
TL36B
Three-eye observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm.
TL36M
Three-eye viewing head with 6.3 megapixel imaging system CMOS camera
TL36P
Three-eye viewing head, equipped with 10.1 inch integrated flat panel display
Eyepiece
Wide-angle eyepiece WF10X,field of view 22mm,optional WF16X,WF25X
Converter
Quadruple Revolving converter
0bjective
Flat field achromatic objective at infinite distance,4X,10X,40X,100X(oil)
Condenser
ABBE N.A.1.25Condenser with Iris Diaphragm &Filter
Stage
Double Layer Mechanical Stage, Size1190X140mm, Moving Range : 60mmx90mm
Focusing
0.02mmCrude motion, micromotion and coaxial focal adjustment system with a minimum focal range of 0.02mm Coarse and Fine Focusing
Light source
3W LED,with Rechargeable Battery,Type C USB cable
Attachments
Dust bag,Type C power cord
Document
TL 36 series biological microscopes are equipped with infinite distance flat field achromatic objective and large field eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, can be widely used in biology, medicine, industry, agriculture and other fields, is the ideal instrument for medical, teaching, scientific research and other units