N-(t-Boc-Aminooxy-PEG2)-N-bis(PEG3-propargyl)

• Real time qPCR kit
• For screening microcystin gene cluster
• Use in combination with Attogene Algae DNA isolation kit

Description

• Description

Attogene’s PCR kit for Microcystin is designed for the In vitro analysis of the MycE gene region responsible for assembling part of the Microcystis peptide. The MycE gene region specific primer and probe mix is provided to be detected through the FAM channel on a qPCR machine. A sample of algae is obtained and washed to extract a clean algal gDNA sample. A reaction mixture is assembled from primers, probe, master mix, and gDNA samples as required. The qPCR machine of choice is set up and loaded as needed and the mixture undergoes PCR amplification. The Primer mix provided exploits the Taq polymerase to amplify the gene region of interest; while the DNA probe mixture is cleaved during amplification to release its FAM fluorophore. The resulting FAM release can be detected on a variety of qPCR platforms.

Real time qPCR kit
For screening microcystin gene cluster
Use in combination with Attogene Algae DNA isolation kit

DBCO-PEG8-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG8-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Name of Product

HybriDetect 2T

Catalog Number

MGHD2 1

Short Info

The HybriDetect 2T is a simple and quick tool to develop your own rapid test. The 2T is designed for simultaneous detection of two analytes. Various molecules can be detected: Proteins, Antibodies, Genetic amplification products

Please note, that you may have to pay country-specific taxes and duties.

Method/Platform

Lateral flow, sandwich or competetive

Range/Assay Sensivity

5 pg DNA, equivalent to agarose gel electrophoresis

Test Principle

HybriDetect 2T is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for simultaneous detection of two different analytes such as proteins, antibodies or gene amplification products. The results can be interpreted qualitative or quantitative.

The HybriDetect 2T is a simple and quick tool to develop your own rapid test. The 2T is designed for simultaneous detection of two analytes. Various molecules can be detected: Proteins, Antibodies, Genetic amplification products