N-(t-butyl ester-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the t-butyl ester can be deprotected and reacted with amines or alcohols to form amides or esters.
N-(t-butyl ester-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the t-butyl ester can be deprotected and reacted with amines or alcohols to form amides or esters.
Description
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis.
Keep Q-PAGE™ Precast Gels flat during storage.
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
| Clone | IHC697 |
| Source | Mouse Monoclonal |
| Positive Control | Colon Cancer |
| Dilution Range | 1:200 |
Thymidylate Synthase (TS) is a crucial enzyme responsible for the synthesis of 2′-deoxythymidine-5′-monophosphate (dTMP) a precursor for thymidylate which is necessary for DNA replication and repair from 2′-deoxyuridine-5′-monophosphate (dUMP). In terms of cancer, TS is an important target for cancer treatment as the inhibition of TS and therefore nucleotide synthesis necessary for cell growth has shown to be a vital part for successful treatment against colorectal, pancreatic and breast cancers.
The Norgen HighRangerPlus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our HighRangerPlus contains nineteen discrete fragments ranging from 100 bp to 10000 bp with higher intensity reference bands at 500 bp, 1000 bp and 5000 bp. This Ladder is ideal for size determination of digested DNA.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Figure 1 / 1
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HighRanger Plus 100 bp DNA Ladder (Cat# 12000) – 100 loads
Ladder Properties:
| Fragment | Size (bp) | Mass (ng) |
| 1 | 10000 | 42 |
| 2 | 8000 | 33 |
| 3 | 6000 | 25 |
| 4 | 5000 | 42 |
| 5 | 4000 | 35 |
| 6 | 3000 | 27 |
| 7 | 2500 | 22 |
| 8 | 2000 | 18 |
| 9 | 1500 | 13 |
| 10 | 1000 | 40 |
| 11 | 900 | 28 |
| 12 | 800 | 25 |
| 13 | 700 | 34 |
| 14 | 600 | 19 |
| 15 | 500 | 44 |
| 16 | 400 | 13 |
| 17 | 300 | 18 |
| 18 | 200 | 13 |
| 19 | 100 | 10 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
This kit is stable for 2 years after the date of shipment.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
