N-(t-butyl ester-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the t-butyl ester can be deprotected and reacted with amines or alcohols to form amides or esters.
N-(t-butyl ester-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the t-butyl ester can be deprotected and reacted with amines or alcohols to form amides or esters.
Cryptococcus neoformans is an encapsulated yeast. Infection with C. neoformans is known as cryptococcosis and is the cause of the most common life-threatening meningitis in patients with weakened immune systems, particularly in advanced HIV/AIDS. C. neoformans is commonly found in soil throughout the world. Human infection of C. neoformans occurs via inhalation of aerosolized spores. From the lungs, C. neoformans is spread hematogenously to the Central Nervous System (CNS), resulting in meningoencephalitis. Although the availability of antiretroviral therapy in the developed world has reduced the incidents of cryptococcosis, it is still a major problem in developing countries and is one of the leading causes of death in patients with HIV/AIDS. In fact, one of the major challenges in treating cryptococcosis is that many patients with cryptococcal CNS disease are asymptomatic in terms of cryptococcal pneumonia, making it difficult for early detection.
Cryptococcus neoformans TaqMan PCR Kit, 100 reactions
Cryptococcus neoformans TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM42750 (100 preps) | Cat. TM42710 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Cryptococcus neoformans Primer & Probe Mix | 280 μL | 280 μL |
| Cryptococcus neoformans Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Usages:
For isolation and cultivation of Listeria monocytogenes.
Principle:
Peptone provide carbon and nitrogen sources; yeast extract powder and starch provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride maintains osmotic equilibrium; glucose carbon source; agar as medium coagulant.
Formulation(per liter):
Trypsin digest of casein 10.0g
Heart trypsin digest 3.0g
Corn starch 1.0g
Pepsin digests meat 5.0g
Yeast extract powder 8.0g
Sodium chloride 5.0g
Glucose 0.5g
Mannitol 10.0g
Ferric ammonium citrate 0.5g
Esculin 0.8g
Lithium chloride 15.0g
Phenol Red 0.1g
Agar 15.0g
Final pH 7.2 ± 0.2
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
250g
Sample type purification kit guide
The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
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| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70100 (24 preps) | Cat. 70110, 70120, 70130, 70140 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V1-V2 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
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