N-(Propargyl-PEG2)-DBCO-PEG3-acid facilitates the creation of triazole linkages with azide-containing compounds using Click Chemistry. The carboxylic group can react with amine-containing entities using coupling agents like HATU for further conjugation.
N-(Propargyl-PEG2)-DBCO-PEG3-acid facilitates the creation of triazole linkages with azide-containing compounds using Click Chemistry. The carboxylic group can react with amine-containing entities using coupling agents like HATU for further conjugation.
This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Kit Contents
| Contents | R662601 | R662602 | R662603 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
| DNase I | 600 μl | 2 x 600 μl | 10 x 600 μl |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| Buffer SPL | 30 ml | 60 ml | 270 ml |
| Buffer PHC | 30 ml | 60 ml | 270 ml |
| Buffer MCB* | 9 ml | 15 ml | 75 ml |
| Buffer ALB3* | 10 ml | 20 ml | 100 ml |
| Buffer GW1* | 44 ml | 66 ml | 2 x 220 ml |
| Buffer RW2* | 20 ml | 50 ml | 2 x 100 ml |
| RNase Free Water | 10 ml | 30 ml | 120 ml |
| 2ml Bead Tubes | 48 | 96 | 5 x 96 |
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
SNPsig® VariPLEX SARSCoV-2 is a real-time-reverse transcriptase PCR (RT-PCR) multiplex assay to be used as a reflex qPCR test. VariPLEX™ SARS-CoV-2 can be performed on extracted sample eluates from positive COVID-19 tests.
Detects the variants originally identified in the UK (20I/501Y.V1), South Africa (20H/501Y.V2), Brazil (20J/501Y.V3) and California (20C/S:452R), and the key biologically significant mutations N501Y and E484K, which are now all prevalent globally.
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