N-(Propargyl-PEG2)-DBCO-PEG3-NHS ester is a PEG linker with a terminal NHS ester to perform facile reactions with amine groups of molecules as well as a propargyl to react with azides to form a triazole. The DBCO can participate in copper-free Click Chemistry reactions.
N-(Propargyl-PEG2)-DBCO-PEG3-NHS ester is a PEG linker with a terminal NHS ester to perform facile reactions with amine groups of molecules as well as a propargyl to react with azides to form a triazole. The DBCO can participate in copper-free Click Chemistry reactions.
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
Specifications
| Features | Specifications |
| Main Functions | Co-isolation DNA and RNA from FFPE tissue |
| Applications | RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc. |
| Purification method | Polydisperse silicon based magnetic beads (DNA)Monodisperse carbonyl magnetic beads (RNA) |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor and pipetting workstation |
| Sample type | FFPE slice, FFPE puncture sample, embedded tissue |
| Sample amount | No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area |
| Yield | DNA: 1 – 10 μg, RNA: 1 – 25 μg |
The sample is lysed and digested under the action of lysate and protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, the supernatant contain RNA was collected and bind to MagBind Particles. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Elution Buffer.
Advantages
Kit Contents
| Contents | R632701 | R632702 | R632703 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagBind Particles | 1.1 ml | 2.5 ml | 11 ml |
| MagPure Particles N | 1.1 ml | 2.5 ml | 11 ml |
| Proteinase K | 24 mg | 48 mg | 220 mg |
| Protease Dissolve Buffer | 3 ml | 10 ml | 15 ml |
| Buffer DPS | 50 ml | 100 ml | 2 x 250 ml |
| Buffer ATL | 20 ml | 30 ml | 120 ml |
| Buffer BST1 | 20 ml | 40 ml | 200 ml |
| Buffer BXW1* | 44 ml | 110 ml | 3 x 110 ml |
| RNase Free Water | 15 ml | 30 ml | 120 ml |
Storage and Stability
Proteinase K, MagPure Particles N and MagBind Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stablefor at least 18 months under these conditions.
Experiment Data
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
Amine-PEG4-Amide-Tri(3-methoxypropanamide-PEG10-Propargyl) Methane HCl salt is a crosslinker consisting of an amino group with three propargyl groups. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research use only.
Amine-PEG4-Amide-Tri(3-methoxypropanamide-PEG10-Propargyl) Methane HCl salt is a crosslinker consisting of an amino group with three propargyl groups. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research use only.
| Clone | IHC008 |
| Source | Mouse Monoclonal |
| Positive Control | Ovarian Carcinoma (Non-Mucinous Carcinoma), Thyroid Carcinoma, Renal Cell Carcinoma |
| Dilution Range | 1:200 |
PAX-8 is a member of the paired box (PAX) family of transcription factors, which are key regulators in early development. This protein plays a role in development of thyroid follicular cells and the expression of thyroid-specific genes, with mutations in the PAX-8 gene linked to thyroid follicular carcinomas, atypical thyroid adenomas, and thyroid dysgenesis. The PAX-8 protein is expressed in simple ovarian inclusion cysts and non-ciliated mucosal cells of the fallopian tubes, but is absent from normal ovarian surface epithelial cells. PAX-8 is also not expressed in normal lung or lung carcinomas. Reports have associated PAX-8 expression with renal carcinoma, nephroblastoma, and seminoma, and have indicated PAX-8 as a useful marker for renal epithelial tumors, ovarian cancer, and for differential diagnoses in lung and neck tumors. Anti-PAX-8 can be useful in determining the primary site of invasive micropapillary carcinomas of ovary from bladder, lung, and breast, when used in adjunct with a panel of organ-specific markers such as uroplakin, mammaglobin, and TTF-1.
