Tri(propargyl-PEG10-NHCO-ethyloxyethyl)amine is a click chemistry branched linker with three terminal azide groups. The hydrophilic PEG spacer increases solubility in aqueous media. The azide group can react with alkyne, BCN, DBCO via Click Chemistry.
Tri(propargyl-PEG10-NHCO-ethyloxyethyl)amine is a click chemistry branched linker with three terminal azide groups. The hydrophilic PEG spacer increases solubility in aqueous media. The azide group can react with alkyne, BCN, DBCO via Click Chemistry.
This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.
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| Kit Specifications | |
| Column Binding Capacity | 50 µg |
| Maximum Column Loading Volume | 650 µL |
| Size of DNA Purified | All sizes |
| Maximum Amount of Starting Material | 1 x 1010 pfu/mL enriched phages |
| Average Yield* | 3-15 µg DNA from 106-1010 pfu/mL of enriched phages |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 46800 (50 preps) | Cat. 46850 (100 preps) |
|---|---|---|
| Lysis Buffer B | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Buffer B | 8 mL | 2 x 8 mL |
| Spin Columns | 50 | 100 |
| Collection Tubes | 50 | 100 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc. Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins, and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
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| Kit Specifications | |
| Maximum Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA < 200 nt |
| Time to Complete 10 Purifications | 50 minutes |
| Average Yield Liver (10mg) Kidney (10mg) Spleen (10mg) Heart (10mg) Muscle (10mg) | 30 μg 25 μg 15 μg 10 μg 5 μg |
| Maximum Amount of Starting Material Heart Kidney Liver Muscle Spleen | 30 mg 15 mg 15 mg 30 mg 15 mg |
Storage Conditions and Product Stability
The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date shipment.
| Component | Cat. 25700 (50 preps) |
|---|---|
| Buffer RL | 30 mL |
| RNase-Free Water | 40 mL |
| Wash Solution A | 38 mL |
| Enzyme Incubation Buffer A | 6 mL |
| Elution Solution A | 6 mL |
| Proteinase K | 2 vials |
| DNase I | 1 vial |
| Mini Spin Columns | 50 |
| Collection Tubes | 100 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
K-FRUGL
SKU: 700004287
110 assays (manual) / 1100 assays (microplate) / 1100 assays (auto-analyser)
| Content: | 110 assays (manual) / 1100 assays (microplate) / 1100 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Fructose, D-Glucose |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 4 to 80 µg of D-glucose, D-fructose or sucrose per assay |
| Limit of Detection: | 0.66 mg/L |
| Reaction Time (min): | ~ 13 min |
| Application examples: | Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread, bakery products, candies, desserts, confectionery, ice-cream, fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals, paper and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by AOAC Method 985.09, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC |
D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See more of our monosaccharide assay kits.
Advantages
D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products.
