PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose.
PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose.
Description
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels Remove tape and comb before electrophoresis.
Keep Q-PAGE™ Precast Gels flat during storage.
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from bacterial culture |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Gram negative or positive bacteria |
| Sample amount | Bacterial culture: 0.5-1.5ml |
| Elution volume | ≥60μl |
| Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
| Contents | D513101 | D513102 | D513103 |
| Purification Times | 48 Preps | 96 Preps | 480 Preps |
| MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
| Lysozyme | 60 mg | 120 mg | 600 mg |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease Dissolve Buffer | 3 ml | 6 ml | 30 ml |
| Buffer SDS | 1.5 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 300 ml |
| Buffer GW1* | 13 ml | 44 ml | 110 ml |
| Buffer TE | 30 ml | 40 ml | 200 ml |
Storage and Stability
MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
For the selective isolation and culture of Listeria monocytogenes.
Peptone provides the carbon and nitrogen sources necessary for growth; yeast extract powder and starch provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride can maintain a balanced osmotic pressure; glucose provides a carbon source; Listeria hydrolyzes esculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; mannitol is a fermentable sugar, and phenol red is a pH indicator; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.
| Ingredients | /liter |
| Peptone | 23 g |
| Starch | 1 g |
| NaCl | 5 g |
| Columbia agar | 13 g |
| Mannitol | 10 g |
| Ferric ammonium citrate | 0.5 g |
| Esculin (aesculin) | 0.8 g |
| Dextrose (glucose) | 0.5 g |
| Lithium chloride | 15.0 g |
| Phenol red | 0.08 g |
| pH7.2±0.2 at 25°C | |
Weigh 68.9g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15min, cool to room temperature and set aside. Heat to dissolve agar before use, cool to 50℃, add 1 tube of supporting reagent (SR0140) per 100mL of basal culture medium, shake well and pour into sterilized culture dish.
The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:
| Quality control strains | Growth |
| Listeria monocytogenes ATCC19115 | Gray-green colonies with a black depression in the center and black surrounding |
| Enterococcus faecalis ATCC29212 | – |
| Escherichia coli ATCC25922 | – |
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Peptone provides the carbon and nitrogen sources necessary for growth; yeast ex……
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