t-Boc-N-amido-PEG4-Amide-Tri-(propargyl-PEG10-ethoxymethyl)-methane

Overview

• Isolate all sizes of circulating and exosomal RNA, including microRNA
• Isolate all sizes of circulating DNA from plasma and serum samples
• Isolate viral and bacterial DNA and RNA
• Versatile plasma and serum input volumes (10 µL – 200 µL)
• No phenol extractions
• Bind and elute all RNA irrespective of size or GC content, without bias
• Concentrate circulating RNA, exosomal RNA and cell-free circulating DNA into a flexible elution volume ranging from 10 µL to 25 µL
• Isolate inhibitor-free nucleic acids
• Purify high-quality RNA and DNA in 30 minutes
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Plasma/Serum RNA/DNA Purification Mini Kit provides a fast, reliable, reproducible and simple procedure for the sequential isolation of circulating RNA, exosomal RNA and Cell-Free Circulating DNA (cfc) from the same Plasma/Serum sample. It can sequentially isolated RNA and DNA from small plasma/serum input ranging from 10 µL to 200 µL. The kit is designed to isolate all sizes of circulating RNA, including microRNA, all sizes of exosomal RNA as well as all sizes of cfc-DNA from fresh or frozen plasma or serum samples. Norgen’s Plasma/Serum RNA Purification Kit provides a clear advantage over other available kits in that it does not require Phenol/Chloroform or any Protease treatments for the isolation of plasma/serum RNA or DNA . RNA and DNA can be eluted into a flexible elution volume ranging from 10 µL to 25 µL. Purified RNA can be used in a number of sensitive downstream applications including reverse transcription qPCR, reverse transcription PCR, NGS, Northern blotting, RNase protection and primer extension, and expression array assays. Purified DNA can be used in a number of sensitive downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.

Background

Typical yields of free-circulating, exosomal RNA and cfc-DNA vary depending on the input sample, as the amount of RNA and/or DNA present in plasma and serum will depend upon the health status of the individual.  Normally, the RNA/DNA yield from plasma or serum is highly variable (range from 1 to 100 ng/mL). Variability is also observed between samples collected from the same donor at different times during the day. This kit is suitable for the isolation of RNA/DNA from serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Details

Supporting Data

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Kit Specifications
Minimum Plasma/Serum Input Volume	10 μL
Maximum Plasma/Serum Input Volume	200 μL
Size of RNA Purified	All sizes including small RNA (<200 nt)
Size of DNA Purified	≥ 50 bp
Minimum Elution Volume	10 μL
Maximum Elution Volume	25 μL
Time to Complete 10 Purifications	15-20 minutes
Average Yield	Variable depending on specimen

 Note: Do not exceed the recommended sample input volume of 200 µL.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Component	Cat. 55200 (50 preps)
Lysis Buffer A	30 mL
Wash Solution A	38 mL
Solution WN	18 mL
Elution Solution A	6 mL
Elution Buffer B	8 mL
Micro Spin Columns	50
Micro-Elute RNA Spin Columns	50
Collection Tube	100
Elution Tubes (1.7 mL)	100
Product Insert	1

Description

Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.

Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.

Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations.  Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.

For the detection of the SARS-CoV-2 variants with the VUI-21APR-01 and VUI-21APR-03 (India)
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target Positive copy number standard curve for quantification Accurate controls to confirm findings 96 reactions, includes master mix

The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.

QPCR is the best method for library quantification. Our reagent only amplifies library molecules that will be used for subsequent emPCR, and is optimized for amplification of various samples. Our reagent is compatible with commercial SYBR Green based QPCR reagents. Quantification of library concentration is achieved by comparison with a standard curve generated from DNA Standards.

The kit comprises DNA Standards (six 10-fold dilutions) and a primer mix.

NGS Library Quantification Standards with PCR Primers (Ion Torrent platform): real time quantitative PCR curve of the standards.

The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.