Tri-(Propargyl-PEG2-ethoxymethyl)-methane-amido-PEG3-carboxylate is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Tri-(Propargyl-PEG2-ethoxymethyl)-methane-amido-PEG3-carboxylate is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-PDCAAS
SKU: 700004324
50 assays per kit
| Content: | 50 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Protein Digestibility |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 570 |
| Signal Response: | Increase |
| Total Assay Time: | ~ 6 h (over 2 days) |
| Application examples: | Protein containing foods and feeds. |
| Method recognition: | Novel Method (Under Patent: U.S. Pat. No. 9,738,920) |
The Protein Digestibility Assay Kit (K-PDCAAS) is used for in vitro measurement of the Animal-Safe Accurate Protein Quality Score (ASAP-Quality Score Method) developed under patent by Medallion Labs. This digestibility score, in conjunction with the essential amino acid profile plus protein and moisture content of the sample, is used to calculate the Protein Digestibility Corrected Amino Acid Score (PDCAAS) value.
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Advantages
The Protein Digestibility Assay Kit (K-PDCAAS) is used for in vitro measurement of the Animal-Safe Accurate Protein Quality Score (ASAP-Quality Score Method) developed under patent by Medallion Labs. This digestibility score, in conjunction with the essential amino acid profile plus protein and moisture content of the sample, is used to calculate the Protein Digestibility Corrected Amino Acid Score (PDCAAS) value.
Norgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants, and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Purification Technology
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.
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| Kit Specifications | |
| Maximum Column Binding Capacity | 1.5 mg |
| Maximum Column Loading Volume | 20 mL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material | |
| Liver Tissue | 100 mg |
| Heart Tissue | 50 mg |
| Kidney Tissue | 100 mg |
| Brain Tissue | 250 mg |
| Lung Tissue | 100 mg |
| Whole Blood | 2 – 10 mL* |
| Bacteria | 2.5 x 10 10 cells |
| Yeast | 2 x 108 cells |
| Fungi | 1 g |
| Plant Tissues | 1 g |
| Time to Complete 4 Purifications | 40 minutes |
| Average Yield: HeLa Cells (5 x 107 cells) E. coli (2.5 x 1010 cells) | ~750 μg ~1.5 mg |
*For isolating total RNA from purified leukocytes
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 26800 (8 preps) |
|---|---|
| Buffer RL | 2 x 40 mL |
| Wash Solution A | 4 x 38 mL |
| Elution Solution A | 3 x 20 mL |
| RNA Maxi Spin Columns (with collection tubes) | 8 |
| Elution Tubes (50 mL) | 8 |
| Product Insert | 1 |
Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) offers a PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations. The kit consists of a specially designed primer mix compatible with the Illumina system, that is used in conjunction with the provided 2X Real-Time PCR Master Mix to amplify a library of unknown concentration. The unknown library is accurately quantified by using a standard curve constructed from the provided DNA Standard (range from 20 pM to 2 fM) on a Real-Time PCR System. The kit is specially optimized to quantify Small RNA-Seq libraries with DNA standards that have similar size to a Small RNA-Seq library. However, it could also be used with other types of NGS libraries.
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Storage Conditions
Upon receipt, store Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
| Component | Cat. 61600 (100 reactions) |
|---|---|
| 2X Real-Time PCR Master Mix | 1 mL |
| NGS Library Quantification Primer Set Mix | 600 µL |
| Quantified NGS Library Standards (for Small-RNA Seq)* | 5 x 80 µL Standards |
| Product Insert | 1 |
* Quantified Library Standards are provided as 20 pM, 2 pM, 200 fM, 20 fM and 2 fM
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