Stool Nucleic Acid Collection and Preservation Tubes

Overview

The preservative provides sample homogeneity eliminating sample variability
Preserve and transport DNA & RNA safely at ambient temperature
Nucleic acid preservation at room temperature over 2 years for DNA and 7 days for RNA
No cold shipping/storage needed – hassle-free and cost effective
Isolate nucleic acids for any application including 16s NGS
Robust preservation over a range of temperatures
Customizable with various accessories for easy and safe collection
Eliminates odor and renders samples safe and non-infectious

Norgen’s Stool Nucleic Acid Collection and Preservation Tubes are designed for the rapid preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Tubes contain Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Stool Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Stool Preservative allow samples to be stored at room temperature for over 2 years for DNA and 7 days for RNA. To extend the stool RNA stability in the preservative, storage at -20°C or -70°C is recommended at the arrival to testing facilities until the RNA purification.

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Supporting Data

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Figure 2. High Integrity of Preserved DNA and RNA

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Kit Specifications
Stool Input	2 g
Stability of Stool Nucleic Acids at Room Temperature	2 years for DNA 7 days for RNA*

* The RNA stability will vary depending on the samples

Storage Conditions and Product Stability

All tubes should be kept tightly sealed and stored at room temperature (15 – 25 °C) for 2 years after the date of shipment without any reduction in performance.

Kit Components	Cat. 45660
Stool Nucleic Acid Collection and Preservation Tubes	50
Product Insert	1

Kit Specifications
Column Binding Capacity	25 µg
Average Yield:* HeLa Cells (1 x 10⁶) Tissue (from 10 mg kidney)	8 µg 10 µg
Maximum Amount of Starting Material: Animal Tissues Cultured Cells Bodily Fluids (blood, saliva) Viral Suspension	20 mg 3 x 10⁶ cells 150 µL 150 µL
Time to Complete 10 Purifications	80 minutes

Component	Cat. 24700 (50 preps)	Cat. 24750 (100 preps)	Cat. 24770 (250 preps)
Digestion Buffer A	25 mL	2 x 25 mL	5 x 25 mL
Buffer SK	30 mL	2 x 30 mL	5 x 30 mL
Wash Solution A	18 mL	2 x 18 mL	5 x 18 mL
Elution Buffer B	30 mL	2 x 30 mL	5 x 30 mL
Proteinase K	12 mg	2 x 12 mg	5 x 12 mg
Spin Columns	50	100	250
Collection Tubes	50	100	250
Elution Tubes (1.7 mL)	50	100	250
Product Insert	1	1	1

Kit Specifications
Maximum Stool Input	200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed	Frozen, fresh or preserved stool
Format	Spin Column
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Elution Volume	50 μL
Time to Complete 10 Purifications	30 minutes
Applications	PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis.

Component	Cat. 27600 (50 preps)	Cat. 65600 (192 preps)	Cat. 55700 (50 preps)	Cat. 63100 (192 preps)
Lysis Buffer L	60 mL	2 x 105 mL	60 mL	3 x 60 mL 1 x 30 mL
Lysis Additive A	6 mL	25 mL	6 mL	25 mL
Binding Buffer I	7 mL	25 mL	7 mL	25 mL
Binding Buffer C	30 mL	110 mL	–	–
Wash Solution A	18 mL	2 x 38 mL	–	–
Magnetic Bead Suspension	–	–	1.1 mL	4 x 1.1 mL
Solution WN	–	–	18 mL	55 mL
Elution Buffer B	8 mL	30 mL	8 mL	30 mL
Bead Tubes	50	200	50	196
Mini Spin Columns	50	–	–	–
96-Well Plate	–	2	–	2
Adhesive Tape	–	4	–	2
Collection Tubes	50	–	–	–
96-Well Collection Plate	–	2	–	–
Elution Tubes (1.7 mL)	50	–	50	–
96-Well Elution Plate	–	2	–	2
Product Insert	1	1	1	1

Features	Specifications
Main Functions	Isolation total DNA from100 mg simple plant
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Economic plant samples
Sample amount	Fresh / frozen plant samples: ≤100mggDried plant / seed samples: ≤20mg
Elution volume	≥30μl
Time per run	30-50 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Stool Nucleic Acid Collection and Preservation Tubes

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Extracting Biological Insights from Stool

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Contents	D316402	D316403
Purification Times	50 Preps	250 Preps
RNase A	10 mg	50 mg
Protease Dissolve Buffer	1.8 ml	5 ml
Buffer SPL	30 ml	150 ml
Buffer PS	10 ml	50 ml
Buffer GW1	22 ml	110 ml
Buffer GW2*	12 ml	2 x 50 ml
Buffer AE	15 ml	60 ml
HiPure gDNA Mini Columns	50	2 x 125
2 ml Collection Tubes	50	2 x 125

Name	CAT NO	Fresh / frozen tissue amount	Dried tissue amount	Column type	Elution volume	Yield (muscle)
HiPure Plant DNA Mini Kit	D3187	150mg	40mg	1.5ml column	50 – 100μl	3-70μg
HiPure HP Plant DNA Maxi Kit	D3163	5g	1g	50ml column	0.7 – 3ml	75-1250μg
HiPure SF Plant DNA Kit	D3164	100mg	20mg	1.5ml column	50 – 100μl	3-50μg
HiPure SF Plant DNA 96 Kit	D3167	50mg	15mg	96 well plate	75 – 150μl	3-30μg