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Stool Nucleic Acid Collection and Preservation Devices Dx

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Detail

Overview

  • CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
  • Ideal for use in in vitro workflows
  • No need to immediately process samples
  • Nucleic acid preservation at room temperature over 2 years for DNA and 7 days for RNA
  • Ship stool samples at room temperature safely
  • Compatible with most DNA/RNA isolation methods 

Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx are designed for the collection and preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Devices Dx – 50 contains 50 individual Stool Nucleic Acid Collection and Preservation Devices Dx. Each Stool Nucleic Acid Collection and Preservation Device Dx consists of Norgen’s Stool Nucleic Acid Collection and Preservation Tube Dx containing Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Nucleic Acid Collection and Preservation Tube Dx is subsequently sent to the laboratory for DNA and/or RNA isolation and analysis. The stool DNA in preserved samples is stable for more than 2 years at room temperature, and the stool RNA in preserved samples is stable for 7 days at room temperature. DNA can be isolated from the preserved stool samples using Norgen’s Stool DNA Isolation Kit Dx (Cat. Dx27600) and RNA can be isolated from the preserved stool samples using Norgen’s Stool Total RNA Purification Kit Dx (Cat. Dx49500). These tubes are ideal for collecting and preserving DNA and RNA samples for in vitro diagnostic use for medical purposes.

NOTE: This product is not available for sale in the United States.

Details

Supporting Data

Figure 1 / 9

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Figure 1. Norgen's Best-In-Class Preservation
Figure 1. Norgen's Best-In-Class Preservation
Figure 2.  High Integrity of Preserved DNA and RNA
Figure 3.  Stability of DNA
Figure 4.  DNA is Stable over a Wide Range of Temperatures
Figure 5. Stability of Genes over a 7 Day Period
Figure 6. Hierarchical Clustering of Stool Samples at Phylum Level
Figure 7. Difference In Top 5 Abundant Microorganisms at Phylum Level
Figure 8. Relative Abundance of Bacterial Genera
Figure 9. Correlation between duplicate bacterial metagenomic reads

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Kit Specifications
Stool Input2 g
Stability of Stool Nucleic Acids at Room Temperature2 years for DNA
7 days for RNA*

* The RNA stability will be vary depending on the samples

Stool Nucleic Acid Collection and Preservation Devices Dx – 50 Contents:

Kit ComponentsCat. Dx45660
Stool Nucleic Acid Collection and Preservation Devices Dx50
Product Insert1

Stool Nucleic Acid Collection and Preservation Device Dx Contents:

Kit ComponentsContents
Stool Nucleic Acid Collection and Preservation Tube Dx filled with preservative1
Insert Card1

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D3163 HiPure HP Plant DNA Maxi Kit

Introduction

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from 3g plant and fungal tissue
ApplicationsPCR, SSR, AFLP, RAPD and southern blot, etc.
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeVarious plant samples (including conventional, polysaccharides and polyphenols)
Sample amountFresh / frozen plant samples: 2-3 gDried plant / seed samples: 0.5-1 g
Elution volume≥500μl
Time per run≤120 minutes
Liquid carrying volume per column20ml
Binding yield of column5mg

Principle

This product is based on silica Column purification. Plant material is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged.DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

Advantages

  • Broad spectrum – suitable for extracting DNA from various plant samples
  • Fast – several samples can be extracted in 30 minutes by silica technology
  • High purity – high quality DNA, completely remove inhibitors
  • High yield – silica technology can achieve the highest yield

Kit Contents

ContentsD316302D316303
Purification Times10 Preps50 Preps
RNase A20 mg90 mg
Protease Dissolve Buffer1.8 ml10 ml
Buffer PAL180 ml900 ml
Buffer GWP150 ml800 ml
Buffer GW225 ml200 ml
Buffer AE30 ml120 ml
HiPure DNA Maxi Columns II1050
50 ml Collection Tubes20100

Storage and Stability

RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Purchase Guide

NameCAT NOFresh / frozen tissue amountDried tissue amountColumn typeElution volumeYield (muscle)
HiPure Plant DNA Mini KitD3187150mg40mg1.5ml column50 – 100μl3-70μg
HiPure HP Plant DNA Maxi KitD31635g1g50ml column0.7 – 3ml75-1250μg
HiPure SF Plant DNA KitD3164100mg20mg1.5ml column50 – 100μl3-50μg
HiPure SF Plant DNA 96 KitD316750mg15mg96 well plate75 – 150μl3-30μg