Overview
- Adenovirus Purification from any input – cell fraction or media fraction
- Rapid purification within 2 to 4.5 hours
- No specialized equipment needed (ultracentrifuge not required)
- Purify adenovirus cell culture supernatant from 1 mL to 33.5 mL input per prep
- Purify adenovirus cell pellet from 1 mL of input per prep
- Up to 25X sample concentration
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Adenoviral vectors are useful tools for both in vitro and in vivo gene transfer, and oncolytic viruses based on adenovirus are highly promising for cancer treatment. Norgen’s Adenovirus Purification Kit provides a fast and simple procedure for concentrating and purifying adenoviral vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. Adenoviral vectors purified in this manner are highly active for use in transduction experiments.
Norgen’s Adenovirus Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit.
Details
Supporting Data
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| Kit Specifications | |
| Resin Binding Capacity (total per kit) | At least 3×1011 adenovirus particles as determined by qPCR At least 3×108 transducing units as determined by transduction assay |
| Input type | Cells, media |
| Input volume (Supernatant) | 1 – 33.5 mL SN per prep (500 mL SN in total) |
| Input Volume (Cell pellet) | 1 mL cell pellet per prep (15 mL in total) |
| Minimum elution volume | 1.3 mL per prep |
| Time to complete purification | 2.5 to 4.5 hours with 1 hour hands on time |
| In vivo transduction | Yes |
Storage Conditions and Product Stability
HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer P should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 67600 (15 preps) |
|---|---|
| Lysis Buffer S | 5.5 mL |
| HL-SAN Nuclease | 102 µL |
| Binding Buffer A | 20 mL |
| Purification Solution C | 60 mL |
| Purification Solution D | 130 mL |
| Wash Solution C | 2 x 130 mL |
| Slurry E | 12.5 mL |
| Elution Buffer P (store at 4°C) | 66 mL |
| Protein Neutralizer | 4 mL |
| Elution tubes (1.7 mL) | 50 |
| Product Insert | 1 |
